TIGIT antibody (AA 28-227)

Catalog No. ABIN1860756

Product Details anti-TIGIT Antibody

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Binding Specificity: AA 28-227

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIGIT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Polyclonal Antibody to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT)

Specificity: The antibody is a rabbit polyclonal antibody raised against TIGIT. It has been selected for its ability to recognize TIGIT in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) corresdonding to Met28~Ser227 (Accession # D3ZTQ2)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Alternative Name: T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT Products)

Synonyms: VSIG9 antibody, VSTM3 antibody, WUCAM antibody, ENSMUSG00000071552 antibody, Vstm3 antibody, RGD1563191 antibody, T-cell immunoreceptor with Ig and ITIM domains antibody, T cell immunoreceptor with Ig and ITIM domains antibody, TIGIT antibody, Tigit antibody

Background: VSIG9, VSTM3, V-Set And Immunoglobulin Domain Containing 9, V-Set And Transmembrane Domain Containing 3

Pathways: Cancer Immune Checkpoints