FLT4 antibody (AA 30-326)

Catalog No. ABIN1860935

Product Details anti-FLT4 Antibody

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Binding Specificity: AA 30-326

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- P TLNITEDSYV IDTGDSLSIS CRGQHPLEWT WPGAQEVLTT GGKDSEDTRV VHDCEGTEAR PYCKVLLLAQ THANNTGSYH CYYKYIKARI EGTTAASTYV FVRDFKHPFI NKPDTLLVNR KDSMWVPCLV SIPGLNITLR SQSSALHPDG QEVLWDDRRG MRVPTQLLRD ALYLQCETTW GDQNFLSNLF VVHITGNELY DIQLYPKKSM ELLVGEKLVL NCTVWAEFDS GVTFDWDYPG KQAERAKWVP ERRSQQTHTE LSSILTIHNV SQNDLGPYVC EANNGIQRFR ESTEVI

Specificity: It has been selected for its ability to recognize VEGFR3 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: VEGFR3 (AA 30-326)

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant VEGFR3 (Pro30~Ile326) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for FLT4

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Alternative Name: Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) (FLT4 Products)

Synonyms: FLT41 antibody, LMPH1A antibody, PCL antibody, VEGFR3 antibody, AI323512 antibody, Chy antibody, Flt-4 antibody, VEGFR-3 antibody, Vegfr3 antibody, cb1059 antibody, fms related tyrosine kinase 4 antibody, FMS-like tyrosine kinase 4 antibody, fms-related tyrosine kinase 4 antibody, FLT4 antibody, Flt4 antibody, flt4 antibody

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling