PUMA antibody (AA 76-170)

Catalog No. ABIN1870134

Product Details anti-PUMA Antibody

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Binding Specificity: AA 76-170

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Polyclonal Antibody to p53 Upregulated Modulator Of Apoptosis (PUMA)

Specificity: The antibody is a rabbit polyclonal antibody raised against PUMA. It has been selected for its ability to recognize PUMA in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant p53 Upregulated Modulator Of Apoptosis (PUMA) corresdonding to Arg76~Val170

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:280-1100 Immunohistochemistry: 5-20 μg/mL

1:28-110 Immunocytochemistry: 5-20 μg/mL

1:28-110 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: p53 Upregulated Modulator Of Apoptosis (BBC3 Products)

Synonyms: BBC3 antibody, Puma antibody, JFY-1 antibody, JFY1 antibody, PUMA antibody, PUMA/JFY1 antibody, BCL2 binding component 3 antibody, Bcl-2 binding component 3 antibody, BBC3 antibody, Bbc3 antibody, bbc3 antibody

Background: JFY1, BBC3, BCL2 Binding Component 3

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity