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Product Details

anti-ATP-binding cassette, subfamily B, member 1 (ABCB1) (Extracellular Epitope) antibody

Overview

Antigen: ATP-binding cassette, subfamily B, member 1 (ABCB1)
Clonality: Monoclonal (MRK16)
Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)
Reactivity: Human
Host: Mouse
2 references available 2 references available
Quantity: 0.15 mg
Price: 820,08 $   (Plus shipping costs and VAT, Invoice in EUR)
Order number: ABIN187222
Availability: Ships within 7 to 10 Business Days
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Additional Information  » anti-ATP-binding cassette, subfamily B, member 1 (ABCB1) (Extracellular Epitope) antibody

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Antigen ATP-binding cassette, subfamily B, member 1 (ABCB1)
Gene ID 5243
Reactivity Human
Clonality Monoclonal
Format Purified
Isotype IgG2a »Matching secondary antibodies
Description The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq] Also known as: CLCS, MDR1, P-GP, PGY1, ABC20, CD243, GP170, MGC163296, ABCB1.
Clone MRK16
Host Mouse

Application Details  » anti-ATP-binding cassette, subfamily B, member 1 (ABCB1) (Extracellular Epitope) antibody

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Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)
» Click here to view 6 available protocols!
Application Notes Potential Uses: cancer multidrug resistance studies, anti-cancer agent screening, AIDS multi-drug resistance studies, nervous system research, immunology research and transplant rejection studies, endocrine/steroid research, endothelium cell and brain P-glycoprotein research. Flow cytometry: For 5 x 105 pelleted cells use 50 µL of 10-100 µg/mL. Immunoprecipitation: Generally use between 25 µg/mL and 50 µg/mL. Immunohistochemistry: Generally use between 0.1 µg/mL and 10 µg/mL. Appropriate dilution must be decided upon according to staining method, conditions, etc. Frozen tissue staining can be done provided that mild fixation treatment is used, such as 4% paraformaldehyde. Acetone, paraffin, methyl alcohol, and ethyl alcohol should not be used as a fixative. Iodine labeling can also be performed on MRK16. Western blot: Does not react. Apparently, the epitope recognized by this antibody is lost in the presence of protein denaturing agents such as SDS. The optimal dilution for a specific application should be determined by the researcher.
Buffer 300 µL of 500 µg/mL monoclonal antibody in PBS, with protein stabilizer, 0.1% sodium azide. Purified by Protein A affinity chromatography. Page 1 of 2
Storage Store at -20 °C. Avoid repeated freeze/thaw cycles.
Restrictions For Research Use only

Publications  » anti-ATP-binding cassette, subfamily B, member 1 (ABCB1) (Extracellular Epitope) antibody

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Publications de Figueiredo-Pontes, Pintao, Oliveira et al.: "Determination of P-glycoprotein, MDR-related protein 1, breast cancer resistance protein, and lung-resistance protein expression in leukemic stem cells of acute myeloid leukemia." In: Cytometry. Part B, Clinical cytometry , Vol. 74, Issue 3, pp. 163-8, 2008 (PubMed)

Valera, de Freitas Cortez, de Paula Queiroz et al.: "Pediatric glioblastoma cell line shows different patterns of expression of transmembrane ABC transporters after in vitro exposure to vinblastine." In: Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery , Vol. 25, Issue 1, pp. 39-45, 2008 (PubMed)