SHOW TUTORIAL VIDEO Show Tutorial Video
Product Details

anti-P-Glycoprotein (ext. Epitope) antibody

Overview

Antigen: P-Glycoprotein (ext. Epitope)
Clonality: Monoclonal (MRK16)
Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)
Reactivity: Human
Host: Mouse
2 references available 2 references available
Quantity: 0.15 mg
Price: 570,00 $   (Plus shipping costs $25 and VAT, Invoice in EUR)
Order number: ABIN187222
Availability: Ships within 3 to 10 Business days
Hints on ordering Hints on ordering
Add to basket

on this page

Additional Information
Application Details
Publications
Related Products
Protocols

Customer Service

Technical Inquiry
Send product details to colleague
Download Datasheet

Additional Information  » anti-P-Glycoprotein (ext. Epitope) antibody

back to top ^


Antigen P-Glycoprotein (ext. Epitope)
Gene ID 5243
Reactivity Human
Clonality Monoclonal
Format Purified
Isotype IgG2a »Matching secondary antibodies
Description The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq] Also known as: CLCS, MDR1, P-GP, PGY1, ABC20, CD243, GP170, MGC163296, ABCB1.
Clone MRK16
Host Mouse

Application Details  » anti-P-Glycoprotein (ext. Epitope) antibody

back to top ^


Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)
» Click here to view 6 available protocols!
Application Notes Potential Uses: cancer multidrug resistance studies, anti-cancer agent screening, AIDS multi-drug resistance studies, nervous system research, immunology research and transplant rejection studies, endocrine/steroid research, endothelium cell and brain P-glycoprotein research. Flow cytometry: For 5 x 105 pelleted cells use 50 µL of 10-100 µg/mL. Immunoprecipitation: Generally use between 25 µg/mL and 50 µg/mL. Immunohistochemistry: Generally use between 0.1 µg/mL and 10 µg/mL. Appropriate dilution must be decided upon according to staining method, conditions, etc. Frozen tissue staining can be done provided that mild fixation treatment is used, such as 4% paraformaldehyde. Acetone, paraffin, methyl alcohol, and ethyl alcohol should not be used as a fixative. Iodine labeling can also be performed on MRK16. Western blot: Does not react. Apparently, the epitope recognized by this antibody is lost in the presence of protein denaturing agents such as SDS. The optimal dilution for a specific application should be determined by the researcher.
Buffer 300 µL of 500 µg/mL monoclonal antibody in PBS, with protein stabilizer, 0.1% sodium azide. Purified by Protein A affinity chromatography. Page 1 of 2
Storage Store at -20 °C. Avoid repeated freeze/thaw cycles.
Restrictions For Research Use only

Publications  » anti-P-Glycoprotein (ext. Epitope) antibody

back to top ^


Publications de Figueiredo-Pontes, Pintao, Oliveira et al.: "Determination of P-glycoprotein, MDR-related protein 1, breast cancer resistance protein, and lung-resistance protein expression in leukemic stem cells of acute myeloid leukemia." In: Cytometry. Part B, Clinical cytometry , Vol. 74, Issue 3, pp. 163-8, 2008 (PubMed)

Valera, de Freitas Cortez, de Paula Queiroz et al.: "Pediatric glioblastoma cell line shows different patterns of expression of transmembrane ABC transporters after in vitro exposure to vinblastine." In: Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery , Vol. 25, Issue 1, pp. 39-45, 2008 (PubMed)