Carbohydrate Antigen 19-9 (CA19-9) antibody

Antigen: Carbohydrate Antigen 19-9 (CA19-9)

Clonality: Monoclonal (KM231)

Host: Mouse

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Western Blotting (WB)

Catalog no.: ABIN187223

Additional Information

Alternative name: CA 19-9

Immunogen: Human gastric cancer cells

Isotype: IgG1

Clone: KM231

Description: Reacts with glycoproteins >1,000 kDa. Reacts strongly with an antigen found in cell membranes and cytoplasm of stomach, colon, breast, and ovarian cancers or lung adenocarcinoma. Reacts weakly with stomach mucosa, intestinal epithelial metaplasia of normal tissue, germ cell or coated epithelium of the colon, acinar cells of the pancreas, epithelium cells of pancreatic duct, gall duct epithelial cells of the liver, renal tubular epithelium of a part of the kidney, bronchus gland of the lung, and part of the bronchus. Does not react with fat, red blood cells nor connective tissues.

Specificity: Reacts with glycoproteins >1,000 kDa. Reacts strongly with an antigen found in cell membranes and cytoplasm of stomach, colon, breast, and ovarian cancers or lung adenocarcinoma. Reacts weakly with stomach mucosa, intestinal epithelial metaplasia of normal tissue, germ cell or coated epithelium of the colon, acinar cells of the pancreas, epithelium cells of pancreatic duct, gall duct epithelial cells of the liver, renal tubular epithelium of a part of the kidney, bronchus gland of the lung, and part of the bronchus. Does not react with fat, red blood cells nor connective tissues.

Application Details

Application Notes: Flow cytometry. Immunohistochemistry: Staining paraffin sections (antigens similar to CA 19-9). Adhesion Blocking: Suggested concentration 1-50 μg/mL. ELISA. HPTLC-immunostaining. RIA. The optimal dilution for a specific application should be determined by the researcher.

Concentration: 200 μg/mL

Buffer: PBS, pH 7.2, with protein stabilizer and 0.1% NaN3.

Storage: Store at -20°C. Aliquot to avoid freeze/thaws.

Research Area: Cancer, Cell/Tissue Markers, Blood Components

Restrictions: For Research Use only

Publications

Publications: Sugawara, Kataoka, Morishita et al.: "Tissue distribution of P-glycoprotein encoded by a multidrug-resistant gene as revealed by a monoclonal antibody, MRK 16." in: Cancer research, Vol. 48, Issue 7, pp. 1926-9, 1988 (PubMed).

Gow, Chinn, Kroetz: "The effects of ABCB1 3'-untranslated region variants on mRNA stability." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 36, Issue 1, pp. 10-5, 2007 (PubMed).

De Rosa, Ackerley, Wang et al.: "Inhibition of multidrug resistance by adamantylgb3, a globotriaosylceramide analog." in: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 4501-11, 2008 (PubMed).

Gow, Hodges, Chinn et al.: "Substrate-dependent effects of human ABCB1 coding polymorphisms." in: The Journal of pharmacology and experimental therapeutics, Vol. 325, Issue 2, pp. 435-42, 2008 (PubMed).

Scheidemann, Loeser, Niedermeier et al.: "The skin as a biofactory for systemic secretion of erythropoietin: potential of genetically modified keratinocytes and fibroblasts." in: Experimental dermatology, Vol. 17, Issue 6, pp. 481-8, 2008 (PubMed).

Crabb, Howell, Rogers et al.: "Characterisation of the in vitro activity of the depsipeptide histone deacetylase inhibitor spiruchostatin A." in: Biochemical pharmacology, Vol. 76, Issue 4, pp. 463-75, 2008 (PubMed).

Candoni, Martinelli, Toffoletti et al.: "Gemtuzumab-ozogamicin in combination with fludarabine, cytarabine, idarubicin (FLAI-GO) as induction therapy in CD33-positive AML patients younger than 65 years." in: Leukemia research, Vol. 32, Issue 12, pp. 1800-8, 2008 (PubMed).

Camera, Rinaldi, Palmieri et al.: "Sequential continuous infusion of fludarabine and cytarabine associated with liposomal daunorubicin (DaunoXome) (FLAD) in primary refractory or relapsed adult acute myeloid leukemia patients." in: Annals of hematology, Vol. 88, Issue 2, pp. 151-8, 2008 (PubMed).

Yang, Wu, Bui et al.: "A novel human multidrug resistance gene MDR1 variant G571A (G191R) modulates cancer drug resistance and efflux transport." in: The Journal of pharmacology and experimental therapeutics, Vol. 327, Issue 2, pp. 474-81, 2008 (PubMed).

Brown, Sayer, Windass et al.: "Characterisation of human tubular cell monolayers as a model of proximal tubular xenobiotic handling." in: Toxicology and applied pharmacology, Vol. 233, Issue 3, pp. 428-38, 2008 (PubMed).