Thymine Dimer antibody
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- Target
- Thymine Dimer
- Reactivity
- Chemical
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- In situ hybridization (ISH)
- Specificity
- Reacts specifically with thymine dimers produced by UV irradiation in double- or single-stranded DNA. Does not react with (6-4) photo products.
- Immunogen
- UV-irradiated salmon sperm DNAMouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) splenocytes.
- Clone
- KTM53
- Isotype
- IgG1
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- Application Notes
- In situ hybridization: Use at a 1:40 to 1:80 dilution. The dilution factor depends on the application. Frozen sections of fixed tissue or formalin-fixed, paraffin-embedded sections are recommended. Southern, Northern and Dot Blot: For the detection of thymine dimers in UV-damaged DNA and thymine-dimerized DNA probes used for in situ. (reported to work, but no data is available) Immunocytochemistry: Reported to work by end-user, protocol available. Immunohistochemistry: Published by user. The optimal dilution for a specific application should be determined by the researcher.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 200 µL of 0.5 mg/mL purified monoclonal antibody in PBS with protein stabilizer and 0.1 % sodium azide. Purified by Protein A chromatography.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- Thymine Dimer
- Target Type
- Chemical
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