anti-Thymine Dimer antibody (HRP)

Overview

Antigen:	Thymine Dimer
Clonality:	Monoclonal (KTM53)
Application:	In situ hybridization (ISH)
Reactivity:	Human, Monkey
Host:	Mouse
Conjugate:	HRP
Quantity:	10 µg
Price:	735,66 $ (Plus shipping costs and VAT, Invoice in EUR)
Order number:	ABIN187268
Availability:	Ships within 7 to 10 Business Days

Antigen	Thymine Dimer
Immunogen	UV-irradiated salmon sperm DNAMouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) splenocytes.
Reactivity	Human, Monkey
Clonality	Monoclonal
Isotype	IgG1 »Matching secondary antibodies
Description	Horseradish peroxidase labeled monoclonal antibody that recognizes thymine dimers in DNA. Labeling ratio (antibody:horseradish peroxidase) = 1:2-1:4.
Clone	KTM53
Host	Mouse
Specificity	Reacts specifically with thymine dimers in double- or single-stranded DNA produced by UV irradiation. Does not react with (6-4) photo products.

Application	In situ hybridization (ISH)
Application Notes	In Situ Hybridization: Use at a 1:40 to 1:80 dilution. The dilution factor depends on the application. Frozen sections of fixed tissue or formalin-fixed, paraffin-embedded sections are recommended. Southern, Northern, and Dot blot: For the detection of thymine dimers in UV-damaged DNA and thymine-dimerized DNA probes. (Reported to work, but no data is available) The optimal dilution for a specific application should be determined by the researcher.
Conjugate	HRP
Buffer	100 µL of 100 µg/mL HRP-labeled monoclonal antibody in PBS, purified by Protein A chromatography. Unlabeled antibody and peroxidase were removed by gel filtration.
Storage	Store at -20 °C. Aliquot to avoid freeze/thaw cycles.
Research Area	DNA/RNA, Cancer
Restrictions	For Research Use only