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Product Details
anti-Thymine Dimer antibody (HRP)
Overview
| Antigen: | Thymine Dimer |
| Clonality: | Monoclonal (KTM53) |
| Application: | In situ hybridization (ISH) |
| Reactivity: | Human, Monkey |
| Host: | Mouse |
| Conjugate: | HRP |
| Quantity: | |
| Price: | 735,66 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN187268 |
| Availability: | Ships within 7 to 10 Business Days |
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Additional Information » anti-Thymine Dimer antibody (HRP)
| Antigen | Thymine Dimer |
| Immunogen | UV-irradiated salmon sperm DNAMouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) splenocytes. |
| Reactivity | Human, Monkey |
| Clonality | Monoclonal |
| Isotype | IgG1 »Matching secondary antibodies |
| Description | Horseradish peroxidase labeled monoclonal antibody that recognizes thymine dimers in DNA. Labeling ratio (antibody:horseradish peroxidase) = 1:2-1:4. |
| Clone | KTM53 |
| Host | Mouse |
| Specificity | Reacts specifically with thymine dimers in double- or single-stranded DNA produced by UV irradiation. Does not react with (6-4) photo products. |
Application Details » anti-Thymine Dimer antibody (HRP)
| Application | In situ hybridization (ISH) |
| Application Notes | In Situ Hybridization: Use at a 1:40 to 1:80 dilution. The dilution factor depends on the application. Frozen sections of fixed tissue or formalin-fixed, paraffin-embedded sections are recommended. Southern, Northern, and Dot blot: For the detection of thymine dimers in UV-damaged DNA and thymine-dimerized DNA probes. (Reported to work, but no data is available) The optimal dilution for a specific application should be determined by the researcher. |
| Conjugate | HRP |
| Buffer | 100 µL of 100 µg/mL HRP-labeled monoclonal antibody in PBS, purified by Protein A chromatography. Unlabeled antibody and peroxidase were removed by gel filtration. |
| Storage | Store at -20 °C. Aliquot to avoid freeze/thaw cycles. |
| Research Area | DNA/RNA, Cancer |
| Restrictions | For Research Use only |

