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Antibody
»
anti-Thymine Dimer HRP
Thymine Dimer antibody (HRP)
| Antigen |
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| Clonality |
Monoclonal (KTM53) |
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Host
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Reactivity
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Human, Monkey
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Conjugate
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HRP
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Application
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| Catalog no. |
ABIN187268 |
| Quantity |
10 µg |
| Price |
550.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Immunogen
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UV-irradiated salmon sperm DNAMouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) splenocytes.
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Isotype
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IgG1
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Clone
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KTM53
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Description
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Horseradish peroxidase labeled monoclonal antibody that recognizes thymine dimers in DNA. Labeling ratio (antibody:horseradish peroxidase) = 1:2-1:4.
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Specificity
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Reacts specifically with thymine dimers in double- or single-stranded DNA produced by UV irradiation. Does not react with (6-4) photo products.
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Application Notes
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In Situ Hybridization: Use at a 1:40 to 1:80 dilution. The dilution factor depends on the application. Frozen sections of fixed tissue or formalin-fixed, paraffin-embedded sections are recommended. Southern, Northern, and Dot blot: For the detection of thymine dimers in UV-damaged DNA and thymine-dimerized DNA probes. (Reported to work, but no data is available) The optimal dilution for a specific application should be determined by the researcher.
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Buffer
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100 µL of 100 µg/mL HRP-labeled monoclonal antibody in PBS, purified by Protein A chromatography. Unlabeled antibody and peroxidase were removed by gel filtration.
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Storage
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Store at -20 °C. Aliquot to avoid freeze/thaw cycles.
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Research Area
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DNA/RNA, Cancer
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Restrictions
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For Research Use only
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Alternatives for antigen "Thymine Dimer", type "Antibodies"
Alternatives 
