Adiponectin, C1Q and Collagen Domain Containing (ADIPOQ) antibody

Antigen: Adiponectin, C1Q and Collagen Domain Containing (ADIPOQ)

Synonyms: ACDC, ADPN, APM1, APM-1, GBP28, ACRP30, ADIPQTL1, adiponectin, APN, Acdc, apM1, 30kDa, adipo, Acrp30, ADIPOQ, MGC138982, ADN, MGC84292

Clonality: Monoclonal (HADI 773)

Host: Mouse

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN187281

Additional Information

Alternative name: Adiponectin

Immunogen: Recombinant human adiponectin.

Isotype: IgG1  (Matching secondary antibodies)

Clone: HADI 773

Specificity: Recognizes both natural and recombinant human adiponectin. Species Reactivity: Human. Others species not tested.

Application Details

Application Notes: ELISA: Direct and indirect, use at a 1:5,000-10,000 dilution. Western blot: Use at a 1:5,000-10,000 dilution with an ECL system. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature. Immunohistochemistry: Paraffin sections. Use at a 1:500 dilution in TBST for 30 minutes at room temperature. The optimal dilution for a specific application should be determined by the researcher. Western blot analysis of human plasma adiponectin with HADI 773 mAb at a 1:5,000 dilution.

Buffer: 50 µg of antibody at 1 mg/mL in PBS, pH 7.4. Contains no preservatives.

Storage: Store at 4 °C short term and at -20 °C long term. Aliquot to avoid freeze/thaw cycles.

Research Area: Cancer, Atherosclerosis, Metabolism, Hormones

Restrictions: For Research Use only