ABCC3 / CMOAT2 / MRP3 antibody

Antigen: ABCC3 / CMOAT2 / MRP3

Clonality: Monoclonal (DTX1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN187297

Additional Information

Immunogen: Recombinant prokaryotic fusion protein corresponding to the cytoplasmic domain, between transmembrane domains 11 and 12, of the MRP3 molecule.

Format: Supernatant

Isotype: IgG2b  (Matching secondary antibodies)

Clone: DTX1

Description: The human multidrug resistance (MDR) -associated protein (MRP) gene family contains at least 6 members designated MRP-1 to 6. MRP3 is a 190 to 200 kDa integral membrane protein which is an organic anion transporter effective in transporting chemotherapeutic drugs such as MTX, etoposide and teniposide. Northern blotting of various human tissues has indicated MRP3 to be expressed in liver, colon, pancreas and at lower levels in the kidney. In liver, MRP3 is localized to the basolateral membrane of hepatocytes and is not associated with the canalicular membrane. MRP3 is also thought to play a role in bile production and secretion in the liver.

Specificity: Recognizes human multidrug resistance-associated protein 3. Species Reactivity: Human. Others not tested. Positive Control: Bowel.

Application Details

Application Notes: Immunohistochemistry: (paraffin sections) Use at a 1:50-1:100 dilution. High temperature antigen unmasking technique using 1 mM EDTA (pH 8.0). Incubation with the primary antibody for 60 minutes at 25oC. Standard ABC technique. Staining Pattern: Membrane with occasional cytoplasmic staining. The optimal dilution for a specific application should be determined by the researcher.

Buffer: Lyophilized tissue culture supernatant containing 15 mM sodium azide. Reconstitute in 1 mL of sterile distilled water.

Storage: Store unopened lyophilized antibody at 4 °C. The reconstituted antibody is stable for at least two months at 4 °C. For long-term storage, store aliquots of the antibody at -20°C to avoid freeze-thaw cycles. Prepare working dilutions on the day of use.

Restrictions: For Research Use only