Bone Morphogenetic Protein 4 (BMP4) antibody

Antigen: Bone Morphogenetic Protein 4 (BMP4)

Synonyms: ZYME, BMP2B, OFC11, BMP2B1, MCOPS6, Bmp-4, Bmp2b, Bmp2b1, Bmp2b-1, BOMPR4A, bmp-4, zbmp-4, MGC100779, zgc:100779, BMP4, BMP-4, xbmp4, XBMP-4, Bmp4, MGC127497, zyme, bmp2b, bmp2b1, bmp4

Clonality: Monoclonal (3H2)

Host: Mouse

Reactivity: Human, Mouse (Murine), Xenopus laevis

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN187303

Additional Information

Alternative name: BMP4

Immunogen: Recombinant mouse BMP4 Staining pattern: Cytoplasmic

Format: Supernatant

Isotype: IgG2b  (Matching secondary antibodies)

Clone: 3H2

Description: Bone morphogenetic protein 4 (BMP4) is one of nine structurally related BMPs belonging to the transforming growth factor beta (TGF-beta) superfamily of secreted proteins. Mature BMP4 is a dimer that binds to a multimeric transmembrane receptor with serine/threonine kinase activity. Although BMP4 was discovered because it stimulates bone formation in adult mammals, it has important roles as a signaling molecule in embryonic tissues including the developing central and peripheral nervous system, musculature and skeleton. It also participates in a signaling pathway found in insects and worms. BMP4 is implicated in fibrodysplasia ossificans progressiva. It is uniquely over-expressed in lymphoblastoid cells and preosseous fibroproliferative lesions. BMPs 1 to 6 are expressed in prostatic adenocarcinoma and BMPs 1 to 5 are expressed in both benign prostatic hyperplasia and ocular melanoma. BMP4 also plays an important role in early tooth morphogenesis.

Specificity: Species Reactivity: Human, mouse and Xenopus. Others not tested. Positive Control: Prostatic adenocarcinoma or osteoclastoma.

Application Details

Application Notes: Western blot Immunohistochemistry: Paraffin sections, Use at a 1:10 - 1:20 dilution. Incubate 60 minutes with primary antibody at 25 °C. Standard ABC technique. The optimal dilution for a specific application should be determined by the researcher.

Buffer: Lyophilized tissue culture supernatant containing 15 mM sodium azide.

Storage: Unopened lyophilized antibody can be stored at 4 °C for short term storage. For long term storage, store at -20 °C. After reconstitution, it is recommended that aliquots of the antibody be made and frozen at -20 °C (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use. Reconstitution: Reconstitute with 1 mL sterile distilled water and let lyophilized pellet dissolve completely. Slight turbidity can occur after reconstitution and does not affect activity. Clarify by centrifugation.

Research Area: Organogenesis, Growth Factors, Angiogenesis, Signaling

Restrictions: For Research Use only