ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 1 (ABCC1) antibody

Antigen: ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 1 (ABCC1)

Synonyms: MRP, ABCC, GS-X, MRP1, ABC29, DKFZp781G125, DKFZp686N04233

Clonality: Monoclonal (MRPr1)

Host: Rat

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN187315

Additional Information

Alternative name: ABCC1 / MRP1

Immunogen: Bacterial fusion protein of MRP containing a segment of 168 amino acids in the amino-proximal half of the protein.Mouse myeloma (SP2/O) x immunized rat (outbred Wistar) lymph node cells

Format: Supernatant

Isotype: IgG2a  (Matching secondary antibodies)

Clone: MRPr1

Specificity: Monoclonal antibody clone MRPr1 reacts with an internal epitope of MRP1, a 180 - 195 kDa transmembrane transporter protein overexpressed in various human non-P-glycoprotein MDR tumor cell lines. No cross-reaction with human MDR1 and MDR3 gene products (P-glycoproteins) or any other MRP. No cross-reaction with any of the human MRP homologues MRP2-6.

Application Details

Application Notes: Can be used for the detection of MRP-related non-Pgp MDR in human tumor samples. Flow Cytometry: Use at least 1:20 - 1:50 dilution after fixing cells in 10% (v/v) FACS Lysing Solution (BD Biosciences cat. no. 349202) in H2O, followed by FITC-conjugated anti-rat IgG secondary antibody. Immunocytochemistry: Acetone-fixed cell preparation at least 1:20 - 1:50. Immunohistochemistry: (Acetone-fixed or frozen sections)- Use at least a 1:20 - 1:50 dilution, followed by incubation with rabbit anti-rat and monoclonal rat APAAP complex. (Formaldehyde-fixed, paraffin-embedded sections)- Use a 1:20 dilution (a 0.01 M citrate pretreatment, 3 x 3 min at 100 °C, may increase the performance of MRPr1), followed by incubation with biotinylated goat anti-rat and streptavidin conjugated to HRP. Western blot: A 1:20 - 1:50 dilution is recommended. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 0.5 ml containing ~250 µg/ml IgG in serum-free culture supernatant with 0.7% BSA and 0.1% NaN3. The antibody solution was filtered through a 0.22 micron filter. Culture medium: RPMI-1640, supplemented with Nutridoma-SR. The medium does not contain serum

Storage: Stable for at least 3 months when stored at 4 °C. Aliquot and store at -20 °C for up to one year. Avoid repeated freeze/thaws.

Research Area: Cancer

Restrictions: For Research Use only

Images

anti-ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 1 (ABCC1) antibody