ABCC2 / CMOAT / MRP2 antibody

Antigen: ABCC2 / CMOAT / MRP2

Clonality: Monoclonal (M2 II-12)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN187320

Additional Information

Immunogen: Bacterial fusion protein containg amino acids 860-950 of human cMOAT/MRP2.

Format: Supernatant

Isotype: IgG2a  (Matching secondary antibodies)

Clone: M2 II-12

Description: Human cMOAT/MRP2 is a 190-200 kD transmembrane protein known as the canalicular multi-organic anion transporter. It is absent in humans with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. cMOAT/MRP2 is closely related to the multidrug resistance-related protein MRP, and cMOAT/MRP2 is overexpressed in a subset of cisplatin-resistant cell lines.

Specificity: Reacts with an internal epitope of human cMOAT/MRP2. Does not cross-react with human MDR1, MRP1, MRP3, and MRP5 gene products. Species Reactivity: Human, others not tested.

Application Details

Application Notes: Western blotting: Use 1:20-50 dilution and anti-mouse HRP. Immunohistochemistry: Use 1:20 on acetone fixed frozen sections can be followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex. Immunocytochemistry: Use 1:20-50 dilution on acetone fixed cytospin preparations. Flow cytometry: Optimal conditions still to be defined. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 0.5 ml of serum free cell culture supernatant concentrated to 250 µg/ml. Contains 0.7% BSA and 0.1% sodium azide.

Storage: Stable for at least 2 months when stored at 2-8 °C, and 1 year at -20 °C. Avoid repeated freeze/thaws.

Restrictions: For Research Use only