Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificity
This clone (P193-6) reacts with an internal epitope (amino acids 593-599, FSKVEDY) or the minor vault protein (VPARP), which is over-expressed in various human non-P-glycoprotein MDR tumor lines, accordingly to an increase in the number of vault particles.
Immunogen
Clone p193-6 was raised against an E. coli lysate transformed with the pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA.
Western blotting: (preferabley with ECL) Use and a dilution of 1:10 to 20. Immunohistochemistry: Use at a dilution of 1:10-20 for staining of paraformaldehyde (4%) fixed cytospin preparations and frozen tissue sections. The following pretreatment is recommended: 10 min with 20 mM glycine in PBS (pH 7.5) and 10 min. with 6 N guanidine hydrochloride in 50 mM Tris-HCL (pH 7.5)1. Optimal conditions for paraffin-embedded tissues have yet to be defined. Flow Cytometry: Optimal conditions have yet to be defined. The optimal dilution for a specific application should be determined by the researcher.
Restrictions
For Research Use only
Format
Liquid
Buffer
This antibody contains approximately 250ug of immunoglobulin per ml. The solution contains 1% BSA and 0.1% sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Target
PARP4
(Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4))