ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 1 (ABCC1) antibody

Antigen: ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 1 (ABCC1)

Synonyms: MRP, ABCC, GS-X, MRP1, ABC29, DKFZp781G125, DKFZp686N04233

Clonality: Monoclonal (MRPr1)

Host: Rat

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN187338

Additional Information

Alternative name: ABCC1 / MRP1

Immunogen: Bacterial fusion protein of MRP containing a segment of 168 amino acids located in the amino-proximal half of the protein.Mouse myeloma (SP2/0) fused with immunized rat (outbred Wistar strain) lymph node cells.

Format: Supernatant

Isotype: IgG2a  (Matching secondary antibodies)

Clone: MRPr1

Specificity: MRPr1 reacts with an epitope of Multidrug Resistance Protein 1 (MRP1, ABCC1), a 180-195 kDa transmembrane transporter protein overexpressed in various human non-P-glycoprotein MDR tumor cell lines. MRPr1 does not cross-react with human MDR1 or MDR3 gene products. Species Reactivity: Human. Others not tested.

Application Details

Application Notes: Flow cytometry: After fixing cells in 10% (v/v) Lysing solution, use MRPr1 at a 1:20-1:50 dilution followed by FITC-conjugated anti-rat IgG secondary antibody. Immunocytochemistry: For acetone-fixed cell preparation use at a 1:20-1:50 dilution. Immunohistochemistry: For acetone-fixed frozen sections, use at a 1:20-1:50 dilution. For formalin-fixed, paraffin-embedded tissues, use at a 1:20 dilution. For paraffin-embedded sections, a 0.01 M citrate pretreatment for 10 min. at 100 °C may increase the performance of MRPr1. Western blot: Use at a 1:20-1:50 dilution, followed by anti-rat-HRP. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 1 mL (>200 tests) or 0.5 mL (>100 tests) monoclonal antibody at ~250 µg IgG/mL in serum-free tissue culture supernatant with protein stabilizer and 0.1% sodium azide. Filtered through a 0.22 µm filter. Culture medium: RPMI-1640, supplemented with Nutrid

Storage: Store at 4 °C for short term. Aliquot and store at -20 °C for long term. Avoid repeated freeze/thaw cycles.

Research Area: Cancer

Restrictions: For Research Use only

Images

anti-ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 1 (ABCC1) antibody

Publications

Publications: Zastre, Chan, Ronaldson et al.: "Up-regulation of P-glycoprotein by HIV protease inhibitors in a human brain microvessel endothelial cell line." in: Journal of neuroscience research, Vol. 87, Issue 4, pp. 1023-36, 2009 (PubMed).

Yin, Huang, Yang et al.: "Characterization and analyses of multidrug resistance-associated protein 1 (MRP1/ABCC1) polymorphisms in Chinese population." in: Pharmacogenetics and genomics, Vol. 19, Issue 3, pp. 206-16, 2009 (PubMed).

Wilson, Fraga, Rodriguez-Galindo et al.: "Expression of the multi-drug resistance proteins and the pregnane X receptor in treated and untreated retinoblastoma." in: Current eye research, Vol. 34, Issue 5, pp. 386-94, 2009 (PubMed).

Peng, Dong, Qi et al.: "A novel two mode-acting inhibitor of ABCG2-mediated multidrug transport and resistance in cancer chemotherapy." in: PLoS ONE, Vol. 4, Issue 5, pp. e5676, 2009 (PubMed).