ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 2 (ABCC2) antibody

Details for Product No. ABIN187342
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Antigen
Synonyms ABC30, CMOAT, DJS, MRP2, cMRP, AI173996, Abc30, Cmoat, Mrp2
Reactivity
Human
(49), (8), (3)
Host
Mouse
(30), (23)
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(51), (23), (22), (16), (15), (14), (11), (3), (2)
Pubmed 7 references available
Quantity 1 mL
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Catalog No. ABIN187342
821.33 $
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Immunogen Bacterial fusion protein of MRP2 containing the 202-amino acid COOH-terminal end of the protein.Mouse myeloma (SP2/0) cells fused with immunized mouse (Balb/c) lymph node cells.
Clone M2 III-6
Isotype IgG2a
Specificity M2III-6 reacts with an internal epitope of human MRP2 (ABCC2), a 170-180 kDa transmembrane protein known as the canalicular multi-organic anion transporter (cMOAT), which is absent in humans with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. MRP2 is closely related to the multidrug resistance-related protein MRP. MRP2 is overexpressed in a subset of cisplatin-resistant cell lines. Does not cross-react with human MDR1, MRP1, MRP3, or MRP5 gene products. Species Reactivity: Reacts with human and rat MRP2. Others not tested.
Purification Supernatant
Sterility 0.22 µm filtered
Alternative Name ABCC2 / CMOAT / MRP2
Application Notes Flow cytometry: Optimal conditions still to be determined. Immunocytochemistry: (Acetone-fixed cyto-spin preparations) Use at a 1:20-1:50 dilution. Immunohistochemistry: (Acetone-fixed frozen sections) Use at a 1:20 dilution, followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex. (Formaldehyde-fixed, paraffin-embedded tissues and tumors) Samples should be pretreated with 0.01 M citric acid (pH 6.0) in distilled water at 100 °C for 10 min. Use MRP2 antibody at a 1:20 dilution. This can be followed by a biotinylated rabbit anti-mouse IgG and streptavidin conjugated to horseradish peroxidase. Western blot: Use at a 1:20-1:50 dilution, followed by anti-mouse HRP. The optimal dilution for a specific application should be determined by the researcher.
Restrictions For Research Use only
Format Liquid
Buffer 1 mL (>200 tests) or 0.5 mL (>100 tests) monoclonal antibody at ~250 µg IgG/mL in serum-free culture supernatant with protein stabilizer and 0.1% sodium azide. Filtered through a 0.22 µm filter. Culture medium: RPMI-1640, supplemented with Nutridoma-SR.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
General Ketabi-Kiyanvash, Herold-Mende, Kashfi et al.: "NKIM-6, a new immortalized human brain capillary endothelial cell line with conserved endothelial characteristics." in: Cell and tissue research, Vol. 328, Issue 1, pp. 19-29, 2007 (PubMed).

Hrebuicek, Jiruasek, Hartmannovua et al.: "Rotor-type hyperbilirubinaemia has no defect in the canalicular bilirubin export pump." in: Liver international : official journal of the International Association for the Study of the Liver, Vol. 27, Issue 4, pp. 485-91, 2007 (PubMed).

Harris, Jeffery: "Sulforaphane and erucin increase MRP1 and MRP2 in human carcinoma cell lines." in: The Journal of nutritional biochemistry, Vol. 19, Issue 4, pp. 246-54, 2008 (PubMed).

Gonzalez, Alvarez, Pisani et al.: "Involvement of oxidative stress in the impairment in biliary secretory function induced by intraperitoneal administration of aluminum to rats." in: Biological trace element research, Vol. 116, Issue 3, pp. 329-48, 2007 (PubMed).

Wang, Boccanfuso, Lemay et al.: "Sex-specific extraction of organic anions by the rat liver." in: Life sciences, Vol. 82, Issue 07. Aug, pp. 436-43, 2008 (PubMed).

Calcagno, Fostel, To et al.: "Single-step doxorubicin-selected cancer cells overexpress the ABCG2 drug transporter through epigenetic changes." in: British journal of cancer, Vol. 98, Issue 9, pp. 1515-24, 2008 (PubMed).

Salphati, Plise, Li: "Expression and activity of the efflux transporters ABCB1, ABCC2 and ABCG2 in the human colorectal carcinoma cell line LS513." in: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, Vol. 37, Issue 03. Apr, pp. 463-8, 2009 (PubMed).

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