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ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 2 (ABCC2) antibody

Details for Product No. ABIN187342, Supplier: Log in to see
Antigen
  • ABC30
  • CMOAT
  • DJS
  • MRP2
  • cMRP
  • AI173996
  • Abc30
  • Cmoat
  • Mrp2
Reactivity
Human
51
7
3
Host
Mouse
27
27
Clonality (Clone)
Monoclonal ()
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
49
22
19
15
11
9
8
4
2
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Immunogen Bacterial fusion protein of MRP2 containing the 202-amino acid COOH-terminal end of the protein.Mouse myeloma (SP2/0) cells fused with immunized mouse (Balb/c) lymph node cells.
Clone M2 III-6
Isotype IgG2a
Specificity M2III-6 reacts with an internal epitope of human MRP2 (ABCC2), a 170-180 kDa transmembrane protein known as the canalicular multi-organic anion transporter (cMOAT), which is absent in humans with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. MRP2 is closely related to the multidrug resistance-related protein MRP. MRP2 is overexpressed in a subset of cisplatin-resistant cell lines. Does not cross-react with human MDR1, MRP1, MRP3, or MRP5 gene products. Species Reactivity: Reacts with human and rat MRP2. Others not tested.
Purification Supernatant
Sterility 0.22 μm filtered
Alternative Name ABCC2 / CMOAT / MRP2 (ABCC2 Antibody Abstract)
Application Notes Flow cytometry: Optimal conditions still to be determined. Immunocytochemistry: (Acetone-fixed cyto-spin preparations) Use at a 1:20-1:50 dilution. Immunohistochemistry: (Acetone-fixed frozen sections) Use at a 1:20 dilution, followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex. (Formaldehyde-fixed, paraffin-embedded tissues and tumors) Samples should be pretreated with 0.01 M citric acid (pH 6.0) in distilled water at 100 °C for 10 min. Use MRP2 antibody at a 1:20 dilution. This can be followed by a biotinylated rabbit anti-mouse IgG and streptavidin conjugated to horseradish peroxidase. Western blot: Use at a 1:20-1:50 dilution, followed by anti-mouse HRP. The optimal dilution for a specific application should be determined by the researcher.
Restrictions For Research Use only
Format Liquid
Buffer 1 mL (>200 tests) or 0.5 mL (>100 tests) monoclonal antibody at ~250 µg IgG/mL in serum-free culture supernatant with protein stabilizer and 0.1% sodium azide. Filtered through a 0.22 µm filter. Culture medium: RPMI-1640, supplemented with Nutridoma-SR.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Background publications Salphati, Plise, Li: "Expression and activity of the efflux transporters ABCB1, ABCC2 and ABCG2 in the human colorectal carcinoma cell line LS513." in: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, Vol. 37, Issue 03. Apr, pp. 463-8, 2009 (PubMed).

Calcagno, Fostel, To et al.: "Single-step doxorubicin-selected cancer cells overexpress the ABCG2 drug transporter through epigenetic changes." in: British journal of cancer, Vol. 98, Issue 9, pp. 1515-24, 2008 (PubMed).

Wang, Boccanfuso, Lemay et al.: "Sex-specific extraction of organic anions by the rat liver." in: Life sciences, Vol. 82, Issue 07. Aug, pp. 436-43, 2008 (PubMed).

Gonzalez, Alvarez, Pisani et al.: "Involvement of oxidative stress in the impairment in biliary secretory function induced by intraperitoneal administration of aluminum to rats." in: Biological trace element research, Vol. 116, Issue 3, pp. 329-48, 2007 (PubMed).

Harris, Jeffery: "Sulforaphane and erucin increase MRP1 and MRP2 in human carcinoma cell lines." in: The Journal of nutritional biochemistry, Vol. 19, Issue 4, pp. 246-54, 2008 (PubMed).

Hrebuicek, Jiruasek, Hartmannovua et al.: "Rotor-type hyperbilirubinaemia has no defect in the canalicular bilirubin export pump." in: Liver international : official journal of the International Association for the Study of the Liver, Vol. 27, Issue 4, pp. 485-91, 2007 (PubMed).

Ketabi-Kiyanvash, Herold-Mende, Kashfi et al.: "NKIM-6, a new immortalized human brain capillary endothelial cell line with conserved endothelial characteristics." in: Cell and tissue research, Vol. 328, Issue 1, pp. 19-29, 2007 (PubMed).