ABCC2 / CMOAT / MRP2 antibody
| Antigen |
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| Clonality |
Monoclonal (M2 II-12) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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| Catalog no. |
ABIN187343 |
| Quantity |
1 ml (Variants) |
| Price |
794.67 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Immunogen
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Bacterial fusion protein containing amino acids 860-950 of human cMOAT/MRP2.Mouse myeloma (SP2/0) x immunized mouse (Balb/c) lymph node cells.
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Format
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Supernatant
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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M2 II-12
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Description
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Human MRP2/cMOAT (ABCC2) is a 190-200 kDa transmembrane protein known as the canalicular multi-organic anion transporter. It is absent in humans with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. MRP2/cMOAT is closely related to the multidrug resistance-related protein MRP, and MRP2/cMOAT is overexpressed in a subset of cisplatin-resistant cell lines.
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Specificity
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Reacts with an internal epitope of human MRP2/cMOAT. Does not cross-react with human MDR1, MRP1, MRP3 and MRP5 gene products. Species Reactivity: Human. Others not tested.
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Application Notes
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Western blotting: Use at a 1:20-50 dilution and anti-mouse HRP. Immunohistochemistry: Use at a 1:20 dilution on acetone fixed frozen sections can be followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex. Immunocytochemistry: Use at a 1:20-50 dilution on acetone fixed cytospin preparations. Flow cytometry: Optimal conditions still to be defined. The optimal dilution for a specific application should be determined by the researcher.
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Buffer
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1 mL or 0.5 mL containing ~250 µg IgG/mL in serum-free culture supernatant with protein stabilizer and 0.1% sodium azide. Filtered through a 0.22 micron filter. Culture medium: RPMI-1640, supplemented with Nutridoma-SR. The medium does not contain serum
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Storage
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Store at 4 °C for short term. Aliquot and store at -20 °C for long term. Avoid repeated freeze/thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "ABCC2 / CMOAT / MRP2", type "Antibodies"
