ABCC3 / CMOAT2 / MRP3 antibody

Antigen: ABCC3 / CMOAT2 / MRP3

Clonality: Monoclonal (M3II-21)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN187352

Additional Information

Immunogen: Bacterial fusion protein containing amino acid residues 830-949 of MRP3.Lymph node cells from an immunized mouse (Balb/C) fused with SP2/0 mouse myeloma cells.

Format: Supernatant

Isotype: IgG2b  (Matching secondary antibodies)

Clone: M3II-21

Specificity: M3II-21 reacts with an internal epitope of human MRP3, a 190-200 kDa transmembrane protein that is closely related to the multi-drug resistance protein MRP1. Does not cross-react with human MDR1, MRP1, MRP2 or MRP5 gene products. Species Reactivity: Human. Others not tested.

Application Details

Application Notes: Flow Cytometry: Optimal conditions still to be defined. Immunocytochemistry: Use at a dilution of 1:20-1:50 on acetone or formaldehyde-fixed cytospin preparations. Immunohistochemistry: Use at a dilution of 1:20 on acetone-fixed frozen sections. Western Blot: Use at a dilution of 1:20-1:50 (tested with anti-mouse HRP). The optimal dilution for a specific application should be determined by the researcher.

Buffer: 1 mL (>200 tests) or 0.5 mL (>100 tests) containing monoclonal antibody in serum free tissue culture supernatant, with protein stabilizer and 0.1% sodium azide. Antibody concentration is approximately 250 µg/mL. Sample has been filtered through a 0.22 µ

Storage: Store at 4 °C for short term. Aliquot and store at -20 °C for long term. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only