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ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (ABCG2) antibody

Antigen

ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (ABCG2)

Synonyms MRX, MXR, ABCP, BCRP, BMDP, MXR1, ABC15, BCRP1, CD338, CDw338, EST157481, MGC102821, Bcrp1, AI428558, ABCG2, MGC127688, bcrp
Clonality Monoclonal (BXP-21)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human

Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Catalog no. ABIN187368
Quantity 1 ml  (Variants)
Price 822.67 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information

Alternative name CD338 / ABCG2 / BCRP1
Immunogen A fusion protein consisting of the E. coli maltose-binding protein and a 126 amino acids part of the BCRP peptide (aa 271-396 of BCRP, GenBank accession no. AF098951).
Isotype IgG2a  (Matching secondary antibodies)
Clone BXP-21
Specificity BXP-21 reacts with an internal epitope of Breast Cancer Resistance Protein (BCRP, ABCG2), a 70 kDa transmembrane half-transporter which is involved in multi-drug resistance. BXP-21 does not cross-react with the human MDR1, MRP1, MRP2 gene products. Species Reactivity: Reacts with human BCRP. Others not tested.

Application Details

Application Notes Immunocytochemistry: Use at a dilution of 1:20-1:50 on acetone-fixed cytospin preparations. Immunohistochemistry: Use at a dilution of 1:20 on acetone-fixed frozen sections, followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex. BXP-21 can be used on formaldehyde-fixed and paraffin-embedded human tissues and tumors: Pretreat with 10 mM citric acid, pH 6.0, for 20 min. Western blot Flow cytometry: Optimal conditions still to be determined.
Buffer 1 mL (>200 tests) or 0.5 mL (>100 tests) monoclonal antibody at ~250 µg IgG/mL in serum-free culture supernatant with protein stabilizer and 0.1% sodium azide.
Storage Store at 4 °C for short term. Aliquot and store at -20 °C for long term. Avoid repeated freeze/thaw cycles. Page 1 of 2
Restrictions For Research Use only

Alternatives

Alternatives for antigen "ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (ABCG2)", type "Antibodies"
Hosts Rabbit (35), Mouse (27), Rat (4)
Reactivities Human (58), Mouse (Murine) (27), Rat (Rattus) (17), Cow (Bovine) (2), Monkey (2), Dog (Canine) (1), Escherichia Coli (E. Coli) (1)
Applications Flow Cytometry (FACS) (40), Western Blotting (WB) (36), Immunofluorescence (IF) (28), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), ELISA (8), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (IHC) (4), Immunocytochemistry (ICC) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (4), PE (3), FITC (2), APC (1), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes C-Term (2), Center (2)