ABCC2 / CMOAT / MRP2 antibody

Antigen: ABCC2 / CMOAT / MRP2

Clonality: Monoclonal (M2 III-5)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN187392

Additional Information

Immunogen: A fusion protein of the bacterial maltose-binding protein and rat Mrp2, containing the 202-amino acid COOH-terminal end of the transporter protein.

Format: Supernatant

Isotype: IgG2b  (Matching secondary antibodies)

Clone: M2 III-5

Specificity: M2III-5 reacts with an internal epitope of MRP2 (ABCC2), a 190-200 kDa transmembrane protein earlier known as the canalicular multi-organic anion transporter (cMOAT), which is absent in humans with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. MRP2 is a member of the MRP family of multidrug resistance related proteins. MRP2 is overexpressed in a subset of cisplatin-resistant cell lines. M2III-5 does not cross-react with the human MDR1 P-gp, MRP1, MRP3 or MRP5 gene products. Species Reactivity: Rat, mouse and human. Others not tested.

Application Details

Application Notes: Immunocytochemistry: Use at a 1:20-1:50 dilution on acetone-fixed cytospin preparations. Immunohistochemistry: Use at a 1:20 dilution on acetone-fixed frozen sections, followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex. M2III-5 can also be used on formaldehyde-fixed paraffin-embedded human tissues and tumors, after pretreatment with 0.01 M citric acid (pH 6.0) in distilled water at 100 °C for 3 x 5 minutes. After incubation with M2III-5 at a 1:20 dilution and washing, slides can be incubated with biotinylated rabbit anti-mouse IgG and streptavidin conjugated to horseradish peroxidase. Western blot: Use at a 1:20-1:50 dilution, followed by anti-mouse HRP. Flow cytometry: Optimal conditions still to be determined. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 1 mL (>200 tests) monoclonal antibody at 250 µg IgG/mL in serum-free culture supernatant with protein stabilizer and 0.1% sodium azide.

Storage: Store at 4 °C for short term. Aliquot and store at -20 °C for long term. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Bochkis, Rubins, White et al.: "Hepatocyte-specific ablation of Foxa2 alters bile acid homeostasis and results in endoplasmic reticulum stress." in: Nature medicine, Vol. 14, Issue 8, pp. 828-36, 2008 (PubMed).