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ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 2 (ABCC2) antibody

Details for Product No. ABIN187392, Supplier: Login to see
Antigen
  • ABC30
  • CMOAT
  • DJS
  • MRP2
  • cMRP
  • AI173996
  • Abc30
  • Cmoat
  • Mrp2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
53
6
2
Host
Mouse
29
27
Clonality (Clone)
Monoclonal ()
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
51
22
21
16
12
11
9
4
2
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Immunogen A fusion protein of the bacterial maltose-binding protein and rat Mrp2, containing the 202-amino acid COOH-terminal end of the transporter protein.
Clone M2 III-5
Isotype IgG2b
Specificity M2III-5 reacts with an internal epitope of MRP2 (ABCC2), a 190-200 kDa transmembrane protein earlier known as the canalicular multi-organic anion transporter (cMOAT), which is absent in humans with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. MRP2 is a member of the MRP family of multidrug resistance related proteins. MRP2 is overexpressed in a subset of cisplatin-resistant cell lines. M2III-5 does not cross-react with the human MDR1 P-gp, MRP1, MRP3 or MRP5 gene products. Species Reactivity: Rat, mouse and human. Others not tested.
Purification Supernatant
Alternative Name ABCC2 / CMOAT / MRP2 (ABCC2 Antibody Abstract)
Pathways
Application Notes Immunocytochemistry: Use at a 1:20-1:50 dilution on acetone-fixed cytospin preparations. Immunohistochemistry: Use at a 1:20 dilution on acetone-fixed frozen sections, followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex. M2III-5 can also be used on formaldehyde-fixed paraffin-embedded human tissues and tumors, after pretreatment with 0.01 M citric acid (pH 6.0) in distilled water at 100 °C for 3 x 5 minutes. After incubation with M2III-5 at a 1:20 dilution and washing, slides can be incubated with biotinylated rabbit anti-mouse IgG and streptavidin conjugated to horseradish peroxidase. Western blot: Use at a 1:20-1:50 dilution, followed by anti-mouse HRP. Flow cytometry: Optimal conditions still to be determined. The optimal dilution for a specific application should be determined by the researcher.
Restrictions For Research Use only
Format Liquid
Buffer 1 mL (>200 tests) monoclonal antibody at 250 µg IgG/mL in serum-free culture supernatant with protein stabilizer and 0.1% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Background publications Bochkis, Rubins, White et al.: "Hepatocyte-specific ablation of Foxa2 alters bile acid homeostasis and results in endoplasmic reticulum stress." in: Nature medicine, Vol. 14, Issue 8, pp. 828-36, 2008 (PubMed).