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ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 2 (ABCC2) antibody

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Synonyms ABC30, CMOAT, DJS, MRP2, cMRP, AI173996, Abc30, Cmoat, Mrp2
Human, Mouse (Murine), Rat (Rattus)
(52), (6), (2)
(29), (26)
Clonality (Clone)
Monoclonal ()
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(50), (21), (21), (15), (12), (11), (9), (4), (2)
Pubmed 1 reference available
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Quantity 1 mL
Shipping to United States ( )
Availability Will be delivered in 11 to 16 Business Days
Immunogen A fusion protein of the bacterial maltose-binding protein and rat Mrp2, containing the 202-amino acid COOH-terminal end of the transporter protein.
Clone M2 III-5
Isotype IgG2b
Specificity M2III-5 reacts with an internal epitope of MRP2 (ABCC2), a 190-200 kDa transmembrane protein earlier known as the canalicular multi-organic anion transporter (cMOAT), which is absent in humans with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. MRP2 is a member of the MRP family of multidrug resistance related proteins. MRP2 is overexpressed in a subset of cisplatin-resistant cell lines. M2III-5 does not cross-react with the human MDR1 P-gp, MRP1, MRP3 or MRP5 gene products. Species Reactivity: Rat, mouse and human. Others not tested.
Purification Supernatant
Alternative Name ABCC2 / CMOAT / MRP2 (ABCC2 Antibody Abstract)
Application Notes Immunocytochemistry: Use at a 1:20-1:50 dilution on acetone-fixed cytospin preparations. Immunohistochemistry: Use at a 1:20 dilution on acetone-fixed frozen sections, followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex. M2III-5 can also be used on formaldehyde-fixed paraffin-embedded human tissues and tumors, after pretreatment with 0.01 M citric acid (pH 6.0) in distilled water at 100 °C for 3 x 5 minutes. After incubation with M2III-5 at a 1:20 dilution and washing, slides can be incubated with biotinylated rabbit anti-mouse IgG and streptavidin conjugated to horseradish peroxidase. Western blot: Use at a 1:20-1:50 dilution, followed by anti-mouse HRP. Flow cytometry: Optimal conditions still to be determined. The optimal dilution for a specific application should be determined by the researcher.
Restrictions For Research Use only
Format Liquid
Buffer 1 mL (>200 tests) monoclonal antibody at 250 µg IgG/mL in serum-free culture supernatant with protein stabilizer and 0.1% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Background publications Bochkis, Rubins, White et al.: "Hepatocyte-specific ablation of Foxa2 alters bile acid homeostasis and results in endoplasmic reticulum stress." in: Nature medicine, Vol. 14, Issue 8, pp. 828-36, 2008 (PubMed).

Catalog No. ABIN187392
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