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APAF1 antibody

APAF1 Reactivity: Human WB, IF, IP, IHC (fro) Host: Rat Monoclonal 2E12 unconjugated
Catalog No. ABIN187407
  • Target See all APAF1 Antibodies
    APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))
    Reactivity
    • 83
    • 51
    • 50
    • 15
    • 1
    Human
    Host
    • 71
    • 8
    • 6
    • 1
    Rat
    Clonality
    • 64
    • 22
    Monoclonal
    Conjugate
    • 42
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This APAF1 antibody is un-conjugated
    Application
    • 51
    • 27
    • 26
    • 26
    • 21
    • 15
    • 14
    • 13
    • 12
    • 10
    • 8
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    Recognizes the CARD domain of human Apaf-1. Species Reactivity: Human, others not tested. Does not cross-react with mouse or rat Apaf-1.
    Immunogen
    FLAG-tagged recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.
    Clone
    2E12
    Isotype
    IgG2a
    Top Product
    Discover our top product APAF1 Primary Antibody
  • Application Notes
    Immunocytochemistry Immunohistochemistry: Electron microscopy on frozen sections. Immunoprecipitation Western blot ELISA The optimal dilution for a specific application should be determined by the researcher.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL.
    Buffer
    100 µg of liquid Protein G purified monoclonal antibody in PBS containing 0.02% sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Target
    APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))
    Alternative Name
    APAF1 (APAF1 Products)
    Synonyms
    APAF1 antibody, ARK antibody, Apaf-1 antibody, Apaf1 antibody, CG6829 antibody, D-Apaf-1 antibody, DARK antibody, Dapaf-1 antibody, Dapaf-1/HAC-1 antibody, Dark antibody, Dark/Apaf-I antibody, Dark/Dapaf-1/HAC1 antibody, Dark/Hac-1/dApaf-1 antibody, Dark/Hac-1/dApaf1 antibody, Dmel\\CG6829 antibody, Hac-1 antibody, Hac-1/Dark antibody, Hac1 antibody, T1 antibody, anon-53Fa antibody, apaf-1 antibody, apaf1 antibody, arc antibody, ark antibody, dAPAF-1 antibody, dApaf-1 antibody, dApaf-1/DARK/HAC-1 antibody, dApaf1 antibody, dArk antibody, dapaf antibody, dapaf-1 antibody, dapaf-1L antibody, dapaf-1S antibody, dark antibody, dark/dapaf-1/hac-1 antibody, dark/hac-1/dapaf-1 antibody, hac-1 antibody, hac1 antibody, l(2)SH0173 antibody, APAF-1 antibody, CED4 antibody, 6230400I06Rik antibody, Apaf1l antibody, fog antibody, mKIAA0413 antibody, zgc:136543 antibody, Death-associated APAF1-related killer antibody, apoptotic peptidase activating factor 1 L homeolog antibody, apoptotic peptidase activating factor 1 antibody, Dark antibody, apaf1.L antibody, apaf1 antibody, APAF1 antibody, Apaf1 antibody
    Background
    Programmed cell death (apoptosis) requires the activation of caspases, which are in turn regulated by adaptor proteins such as Apaf-1 (activator of apoptosis protease-activating factor-1). The 136 kDa protein Apaf-1 is a mammalian homolog of C. elegans/CED-4, and is required for some pathways of cell death. Apaf-1 promotes the aggregation and self-processing of the initiator caspase, caspase-9 in the presence of cytochrome c and dATP. It consists of four function-determining regions: (1) an N-terminal caspase activation and recruitment domain (CARD), which is required for binding to pro-caspase-9, (2) a central CED-4 homologous region, (3) an Apaf-1 specific region, and (4) 12 identified WD40 repeats at the C-terminus of the protein.
    Pathways
    p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity
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