X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1) antibody

Antigen: X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1)

Synonyms: RCC, Xrcc-1, MGC102556, xrcc1, MGC80909, XRCC1

Clonality: Monoclonal (33-2-5)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Application: Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN187442

Additional Information

Alternative name: XRCC1

Immunogen: Recombinant human XRCC1 protein.

Isotype: IgG2b  (Matching secondary antibodies)

Clone: 33-2-5

Description: X-Ray Repair Cross Complementing (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNA ligase III through the C-terminal 96 amino acids and to DNA polymerase beta through the N-terminal half. In the testis XRCC1 is expressed at high levels. Cells with a mutation of this gene exhibit decreased single strand break repair and reduced recombination repair. They show increased double strand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 might serve as a scaffold protein during base excision-repair.

Specificity: This clone recognizes a protein of approximately 85 kDa identified as the XRCC1. Species Reactivity: Human and rat. Others not tested. Molecular Weight: .85 kDa Cellular Location: Nuclear.

Application Details

Application Notes: Immunoprecipitation: (Native verified) Use Protein A. Use antibody at 2 µg/mg protein lysate. Immunohistochemistry: (Formalin/paraffin) Use antibody at 1-2 µg/mL for 30 min. at RT. [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 min.] Western blot: Use antibody at 1-2 µg/mL for 2 hrs. at RT. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 100 µg of monoclonal antibody at 200 µg/mL in 10 mM PBS, pH 7.4, protein stabilizer and 0.09% sodium azide. Purified from ascites fluid by Protein A chromatography.

Storage: Store at 4 °C Positive Controls: Ls174T, HT29 and HeLa cells. Normal testis.

Research Area: Cancer, DNA/RNA

Restrictions: For Research Use only

Images

anti-X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1) antibody

anti-X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1) antibody (Image 2)

Publications

Publications: Artwohl, Lindenmair, Roden et al.: "Fatty acids induce apoptosis in human smooth muscle cells depending on chain length, saturation, and duration of exposure." in: Atherosclerosis, Vol. 202, Issue 2, pp. 351-62, 2009 (PubMed).