Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5) antibody

Antigen: Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5)

Synonyms: CEA, CD66e, DKFZp781M2392, Psg30, 1600029H12Rik, CEACAM5, CEACAM

Clonality: Monoclonal (COL-1)

Host: Mouse

Reactivity: Human

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN187497

Additional Information

Alternative name: CD66e / CEACAM5

Immunogen: Human colon carcinoma extract

Isotype: IgG2a

Clone: COL-1

Description: Clone COL-1 has a high affinity to carcinoembryonic antigen (CEA) and shows no detectable reactivity to biliary glycoprotein (BPG), nonspecific cross-reacting antigen (NCA) or human polymorphonuclear leucocytes. This antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). CEA is not found in benign glands, stroma or malignant prostatic cells. This antibody shows no reaction with a variety of normal tissues.

Specificity: Species Reactivity: Human. Does not react with rat. Other species not tested. Molecular Weight: .180 kDa Cellular Location: Cytoplasmic and lumenal membrane.Positive Control: LS174T cells, colon carcinoma.

Application Details

Application Notes: Immunofluorescence Immunoprecipitation: Native and denatured. Use antibody at 2 µg/mg protein lysate in conjunction with Protein A. Western blot: Use antibody at 1-2 µg/mL for 2 hours at room temperature. Immunohistochemistry: Formalin/paraffin, use at a 1:1,000 dilution for 30 minutes at room temperature. Staining of formalin-fixed tissue REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling for 20 minutes at room temperature. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 1 mL of 200 µg/mL of monoclonal antibody purified from the ascites fluid by Protein A chromatography. Prepared in 10 mM PBS, pH 7.4, with protein stabilizer and 0.09% sodium azide.

Storage: Store at 4 °C.

Research Area: Cancer, Receptors, Immunology, Cell/Tissue Markers, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only