CASP8 and FADD-Like Apoptosis Regulator (CFLAR) antibody

Antigen: CASP8 and FADD-Like Apoptosis Regulator (CFLAR)

Synonyms: CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, Cash, Flip, Gm9845, c-Flip, AI646576, AU021929, MGC103395, 2310024N18Rik, A430105C05Rik, ENSMUSG00000072980, C-FLIP, MGC108616, Clarp1, CFLAR, DKFZp469E1815

Clonality: Monoclonal (Dave-2)

Host: Rat

Reactivity: Human, Mouse (Murine)

Application: Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN187515

Additional Information

Alternative name: CFLAR / Casper / I-FLICE

Immunogen: Recombinant human FLIP (aa 1-480)

Isotype: IgG2a  (Matching secondary antibodies)

Clone: Dave-2

Description: Apoptosis is linked to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family. Caspase-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by death receptors CD95, DR3, TRAIL-R and TNFR1.

Specificity: Recognizes FLIP. The epitope recognized (amino acids 1-200) is present in both the long (FLIPL) and short (FLIPS) splice variants. Species Reactivity: Human and mouse. Others not tested.

Synonyms: CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, Cash, Flip, Gm9845, c-Flip, AI646576, AU021929, MGC103395, 2310024N18Rik, A430105C05Rik, ENSMUSG00000072980, C-FLIP, MGC108616, Clarp1, CFLAR, DKFZp469E1815

Application Details

Application Notes: Immunoprecipitation Western blot Immunohistochemistry: NOT RECOMMENDED. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 100 µg of liquid purified monoclonal antibody at 1 mg/mL in PBS containing 10% glycerol and 0.02% sodium azide.

Storage: For long term storage, aliquot and store at -20 °C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) antibody