T Cell Factor 3/4 (TCF3/4) antibody

Antigen: T Cell Factor 3/4 (TCF3/4)

Clonality: Monoclonal (6F12-3)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN187660

Additional Information

Immunogen: His-tagged fusion protein corresponding to amino acids 31-331 of the human TCF.

Isotype: IgG  (Matching secondary antibodies)

Clone: 6F12-3

Description: TCF-3 and TCF-4 are members of the TCF/LEF (T cell factor / lymphoid enhancer factor) family of transcription factors. Members of this family along with beta catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF-4 generates transcriptionally active complexes with beta catenin in colon carcinoma cells. Expression of TCF-4 is highest in the CNS and intestinal epithelium where Wnt signaling also plays an essential role in development. Aberrant TCF-4 transcription activity has been implicated in carcinogenesis of intestinal and mammary epithelia. TCF-3 appears to be expressed exclusively of TCF-4 in the hair follicles, stomach epithelium and keratinocytes of the skin.

Specificity: Monoclonal antibody against TCF-3 and TCF-4. Species Reactivity: Mouse and human. Others not tested.

Application Details

Application Notes: Western blot: Detects TCF-4 at 1-4 µg/mL. Detects a 60 kDa band in Cos-1 cells transfected with human TCF-3. Immunohistochemistry: paraffin embedded tissue section staining. Gel supershift assays The optimal dilution for a specific application should be determined by the researcher.

Buffer: Provided as 0.2 µm sterile filtered solution in 0.1 M Tris-glycine, pH 7.4, 0.150 M NaCl, 30% glycerol with 0.08% sodium azide. See vial label for the lot specific concentration.

Storage: Stored at -20 °C. Aliquot to avoid freeze/thaw cycles.

Restrictions: For Research Use only