ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (ABCG2) antibody

Antigen: ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (ABCG2)

Synonyms: MRX, MXR, ABCP, BCRP, BMDP, MXR1, ABC15, BCRP1, CD338, CDw338, EST157481, MGC102821, Bcrp1, AI428558, ABCG2, MGC127688, bcrp

Clonality: Monoclonal (BXP-53)

Host: Rat

Reactivity: Human, Mouse (Murine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN187694

Additional Information

Alternative name: CD338 / ABCG2 / BCRP1

Immunogen: A fusion protein containing a fragment of the mouse Bcrp protein corresponding to amino acids 221-394 and E. coli maltose-binding protein.

Format: Supernatant

Isotype: IgG2a  (Matching secondary antibodies)

Clone: BXP-53

Specificity: BXP-53 reacts with an internal epitope of Breast Cancer Resistance Protein (Bcrp, Abcg2), a 70 kDa transmembrane half-transporter which is involved in multidrug resistance. BXP-53 also reacts with the human BCRP molecule. Species Reactivity: Human and mouse. Others not tested.

Application Details

Application Notes: Immunocytochemistry: Use at a 1:20-1:50 dilution on acetone-fixed cytospin preparations. Immunohistochemistry: Use at a 1:20 dilution on acetone-fixed frozen sections, followed by incubation with biotin-labeled rabbit anti-rat IgG and HRP-labeled streptavidin. Western blot: Use at a 1:20-1:50 dilution, followed by HRP-labeled anti-rat IgG. Flow cytometry: Optimal conditions still to be determined. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 1 mL (>200 tests) monoclonal antibody at ~250 µg IgG/mL in serum-free culture supernatant with protein stabilizer and 0.1% sodium azide.

Storage: Store at 4 °C for short term. Aliquot and store at -20 °C for long term. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Lee, Babakhanian, Ramaswamy et al.: "Expression of the ATP-binding cassette membrane transporter, ABCG2, in human and rodent brain microvessel endothelial and glial cell culture systems." in: Pharmaceutical research, Vol. 24, Issue 7, pp. 1262-74, 2007 (PubMed).

Roberts, Black, Raman et al.: "Subcellular localization of transporters along the rat blood-brain barrier and blood-cerebral-spinal fluid barrier by in vivo biotinylation." in: Neuroscience, Vol. 155, Issue 2, pp. 423-38, 2008 (PubMed).

Merino, Real, Baro et al.: "Natural allelic variants of bovine ATP-binding cassette transporter ABCG2: increased activity of the Ser581 variant and development of tools for the discovery of new ABCG2 inhibitors." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 37, Issue 1, pp. 05. Sep, 2008 (PubMed).

Ifergan, Goler-Baron, Assaraf: "Riboflavin concentration within ABCG2-rich extracellular vesicles is a novel marker for multidrug resistance in malignant cells." in: Biochemical and biophysical research communications, Vol. 380, Issue 1, pp. 05. Okt, 2009 (PubMed).

Zhao, Raub, Sawada et al.: "Breast cancer resistance protein interacts with various compounds in vitro, but plays a minor role in substrate efflux at the blood-brain barrier." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 37, Issue 6, pp. 1251-8, 2009 (PubMed).

Lemos, Kathmann, Giovannetti et al.: "Impact of cellular folate status and epidermal growth factor receptor expression on BCRP/ABCG2-mediated resistance to gefitinib and erlotinib." in: British journal of cancer, Vol. 100, Issue 7, pp. 1120-7, 2009 (PubMed).

Bisson, Prowse: "WNT signaling regulates self-renewal and differentiation of prostate cancer cells with stem cell characteristics." in: Cell research, Vol. 19, Issue 6, pp. 683-97, 2009 (PubMed).

Lee, Abe, Bridges et al.: "Sex-dependent Disposition of Acetaminophen Sulfate and Glucuronide in the In Situ Perfused Mouse Liver." in: Drug metabolism and disposition: the biological fate of chemicals, 2009 (PubMed).