ADAM Metallopeptidase Domain 10 (ADAM10) antibody

Antigen: ADAM Metallopeptidase Domain 10 (ADAM10)

Synonyms: kuz, AD10, MADM, CD156c, HsT18717, kuzbanian, 1700031C13Rik, ADAM10, adam10, wu:fc03d12, ADAM-10, DKFZp459A157, LOC100219653, Adam10

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN187754

Additional Information

Alternative name: CD156c / ADAM10

Immunogen: A peptide corresponding to amino acids 732 to 748 of human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid.

Description: Proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-alpha, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metallo-protease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-alpha, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

Specificity: This antibody recognizes ADAM10. Species Reactivity: Human, mouse, and rat. Other species not tested. Positive Control: Jurkat whole cell lysate. An approximately 85 kDa band, which may represent precursor, can be detected. Figure 1. Western blot analysis of ADAM10 in Jurkat whole cell lysate in the absence (1) or presence (2) of blocking peptide with anti-ADAM10 at 1 µg/mL. Figure 2. Immunohistochemical staining of K562 cells using anti-ADAM10 at 2 µg/mL.

Application Details

Application Notes: Immunohistochemistry: Use at 2 µg/mL. Western blot: Use at a 1:500-1:2,000 dilution. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 200 µL at 500 µg/mL polyclonal antibody in PBS containing 0.02% sodium azide. Purified by ion exchange chromatography.

Storage: Store at 4 °C.

Research Area: Proteases, Extracellular Matrix

Restrictions: For Research Use only