X-Linked Inhibitor of Apoptosis (XIAP) antibody

Antigen: X-Linked Inhibitor of Apoptosis (XIAP)

Synonyms: API3, ILP1, MIHA, XLP2, BIRC4, XIAP, birc4, xiap, MGC107868, xxiap

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN187918

Additional Information

Alternative name: IAP3 / BIRC4

Immunogen: Synthetic peptide corresponding to 13 amino acids at the C-terminus of human XIAP (Genbank accession No. NP_001158).

Description: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases . The X-chromosome linked inhibitor of apoptosis (XIAP) contains 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7, and -9. Upregulation of XIAP expression can protect cells from apoptosis induced by low level radiation, conversely, decreased XIAP expression by antisense targeting resulted in increased cell death following low level radiation. Two negative regulators, termed XAF-1 and Smac, can bind and inhibit XIAP activity.

Specificity: Recognizes XIAP. Species Reactivity: Human and mouse. Other species not tested. Positive Control: Human kidney cell lysate.

Application Details

Application Notes: Immunohistochemistry: Use 2.0 µg/mL. Western blot: Recommend 0.5-2.0 µg/mL. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 100 µg in 100 µL of PBS containing 0.02% sodium azide. Immunoaffinity purified IgG.

Storage: Store at 4 °C.

Research Area: Cancer, Apoptosis/Necrosis

Restrictions: For Research Use only