RFWD2 antibody (C-Term)

Catalog No. ABIN1881747

Product Details anti-RFWD2 Antibody

Target: RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))

Binding Specificity: AA 672-701, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFWD2 antibody is un-conjugated

Application: Western Blotting (WB)

Predicted Reactivity: M

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This RFWD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 672-701 amino acids from the C-terminal region of human RFWD2.

Clone: RB41831

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-RFWD2 antibody (ABIN1881747)

Shimada, Miyagawa, Kawashima, Tanaka, Honda, Honda, Tokunaga: "An approach based on a genome-wide association study reveals candidate loci for narcolepsy." in: Human genetics, Vol. 128, Issue 4, pp. 433-41, (2010) (PubMed).

Li, Ohshiro, Reddy, Pakala, Lee, Zhang, Rayala, Kumar: "E3 ubiquitin ligase COP1 regulates the stability and functions of MTA1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 41, pp. 17493-8, (2009) (PubMed).

Kato, Ding, Pisck, Jhala, Du: "COP1 functions as a FoxO1 ubiquitin E3 ligase to regulate FoxO1-mediated gene expression." in: The Journal of biological chemistry, Vol. 283, Issue 51, pp. 35464-73, (2008) (PubMed).

Target Details for RFWD2

Target: RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))

Alternative Name: RFWD2 (RFWD2 Products)

Synonyms: COP1 antibody, RNF200 antibody, AI316802 antibody, C80879 antibody, Cop1 antibody, RGD1304773 antibody, RFWD2 antibody, cop1 antibody, rnf200 antibody, zgc:163067 antibody, ring finger and WD repeat domain 2 antibody, ring finger and WD repeat domain 2, E3 ubiquitin protein ligase antibody, ring finger and WD repeat domain 2, E3 ubiquitin protein ligase L homeolog antibody, RFWD2 antibody, Rfwd2 antibody, rfwd2 antibody, rfwd2.L antibody

Background: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.

Molecular Weight: 80474

NCBI Accession: NP_001001740, NP_071902

UniProt: Q8NHY2

Pathways: Photoperiodism