UBE2S antibody (C-Term)

Catalog No. ABIN1881968

Product Details anti-UBE2S Antibody

Target: UBE2S (Ubiquitin-Conjugating Enzyme E2S (UBE2S))

Binding Specificity: AA 183-210, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2S antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: X, B, M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This UBE2S antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-210 amino acids from the C-terminal region of human UBE2S.

Clone: RB42367

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-UBE2S antibody (ABIN1881968)

Wu, Merbl, Huo, Gallop, Tzur, Kirschner: "UBE2S drives elongation of K11-linked ubiquitin chains by the anaphase-promoting complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 4, pp. 1355-60, (2010) (PubMed).

Williamson, Wickliffe, Mellone, Song, Karpen, Rape: "Identification of a physiological E2 module for the human anaphase-promoting complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 43, pp. 18213-8, (2009) (PubMed).

Chen, Lee, Lu, Chen, Hsieh, Liu, Lin, Chen: "The predictive role of E2-EPF ubiquitin carrier protein in esophageal squamous cell carcinoma." in: Journal of molecular medicine (Berlin, Germany), Vol. 87, Issue 3, pp. 307-20, (2009) (PubMed).

Garnett, Mansfeld, Godwin, Matsusaka, Wu, Russell, Pines, Venkitaraman: "UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit." in: Nature cell biology, Vol. 11, Issue 11, pp. 1363-9, (2009) (PubMed).

Lim, Jung, Lee, Im: "Egr-1 and serum response factor are involved in growth factors- and serum-mediated induction of E2-EPF UCP expression that regulates the VHL-HIF pathway." in: Journal of cellular biochemistry, Vol. 105, Issue 4, pp. 1117-27, (2008) (PubMed).

Target Details for UBE2S

Target: UBE2S (Ubiquitin-Conjugating Enzyme E2S (UBE2S))

Alternative Name: UBE2S (UBE2S Products)

Background: This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.

Molecular Weight: 23845

NCBI Accession: NP_055316

UniProt: Q16763

Pathways: Ubiquitin Proteasome Pathway