Topoisomerase I antibody (N-Term)
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- Target See all Topoisomerase I (TOP1) Antibodies
- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Topoisomerase I antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS)
- Purification
- This antibody is purified through a protein G column, followed by dialysis against PBS.
- Immunogen
- This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.
- Clone
- 1291CT875-142-166
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product TOP1 Primary Antibody
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- Application Notes
- WB: 1:1000. FC: 1:25
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Expiry Date
- 6 months
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- Target
- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
- Alternative Name
- TOP1 (TOP1 Products)
- Synonyms
- CG6146 antibody, Dmel\\CG6146 antibody, TOP1 antibody, TopI antibody, Topo I antibody, TopoI antibody, dTopoI antibody, l(1)G0134 antibody, l(1)G0201 antibody, l(1)G0229 antibody, l(1)G0278 antibody, l(1)top1 antibody, top1 antibody, topo I antibody, topoI antibody, DNA topoisomerase 1 beta antibody, DNA topoisomerase I alpha antibody, MGO1 antibody, MGOUN 1 antibody, MTE17.1 antibody, MTE17_1 antibody, TOP1BETA antibody, TOPOISOMERASE 1 antibody, TOPI antibody, AI467334 antibody, D130064I21Rik antibody, Top-1 antibody, Ab2-086 antibody, topi antibody, Topoisomerase 1 antibody, DNA topoisomerase I antibody, DNA topoisomerase I alpha antibody, DNA topoisomerase 1 antibody, topoisomerase (DNA) I antibody, DNA topoisomerase I, gene 1 L homeolog antibody, Top1 antibody, top1 antibody, topA antibody, ZMO1193 antibody, MRAD2831_RS63095 antibody, Lferr_0016 antibody, PC1_2015 antibody, Za10_0136 antibody, Gbro_0575 antibody, Dd586_2108 antibody, Kvar_3044 antibody, Alvin_2069 antibody, Dacet_0849 antibody, Mrub_1615 antibody, Arnit_2962 antibody, Ndas_4953 antibody, Trad_2377 antibody, Acear_1653 antibody, Mahau_0844 antibody, Mesop_4109 antibody, Thein_1586 antibody, Theth_0241 antibody, TOP1ALPHA antibody, top-1 antibody, TOP1 antibody, top1.1.L antibody
- Target Type
- Viral Protein
- Background
- Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
- Molecular Weight
- 90726
- UniProt
- P11387
- Pathways
- Caspase Cascade in Apoptosis, Stem Cell Maintenance
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