TOLLIP antibody (C-Term)

Catalog No. ABIN1882142

Product Details anti-TOLLIP Antibody

Target: TOLLIP (Toll Interacting Protein (TOLLIP))

Binding Specificity: AA 221-250, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOLLIP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This TOLLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human TOLLIP.

Clone: RB4409

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-TOLLIP antibody (ABIN1882142)

Chen, Choong, Lin, Philp, Wong, Ang, Tan, Loh, Hew, Shah, Druker, Chong, Lim: "Differential expression of novel tyrosine kinase substrates during breast cancer development." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 12, pp. 2072-87, (2007) (PubMed).

Zhang, Ghosh: "Negative regulation of toll-like receptor-mediated signaling by Tollip." in: The Journal of biological chemistry, Vol. 277, Issue 9, pp. 7059-65, (2002) (PubMed).

Bulut, Faure, Thomas, Equils, Arditi et al.: "Cooperation of Toll-like receptor 2 and 6 for cellular activation by soluble tuberculosis factor and Borrelia burgdorferi outer surface protein A lipoprotein: role of Toll-interacting protein and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 2, pp. 987-94, (2001) (PubMed).

Burns, Clatworthy, Martin, Martinon, Plumpton, Maschera, Lewis, Ray, Tschopp, Volpe: "Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor." in: Nature cell biology, Vol. 2, Issue 6, pp. 346-51, (2000) (PubMed).

Volpe, Clatworthy, Kaptein, Maschera, Griffin, Ray: "The IL1 receptor accessory protein is responsible for the recruitment of the interleukin-1 receptor associated kinase to the IL1/IL1 receptor I complex." in: FEBS letters, Vol. 419, Issue 1, pp. 41-4, (1998) (PubMed).

Target Details for TOLLIP

Target: TOLLIP (Toll Interacting Protein (TOLLIP))

Alternative Name: TOLLIP (TOLLIP Products)

Synonyms: IL-1RAcPIP antibody, 4930403G24Rik antibody, 4931428G15Rik antibody, fk22a02 antibody, im:7145391 antibody, wu:fk22a02 antibody, zgc:76985 antibody, TOLLIP antibody, GB17961 antibody, tollip antibody, tollip1 antibody, tollipa antibody, TOLIP antibody, toll interacting protein antibody, toll-interacting protein antibody, toll-interacting protein-like antibody, toll interacting protein L homeolog antibody, toll interacting protein S homeolog antibody, Toll-interleukine I receptor interacting protein I antibody, TOLLIP antibody, Tollip antibody, tollip antibody, LOC552034 antibody, LOC100169858 antibody, tollip.L antibody, tollip.S antibody, LOC100136122 antibody

Background: Toll like protein is a component of the signaling pathway of IL1 and Toll like receptors. It inhibits cell activation by microbial products. Tollip recruits IRAK1 to the IL1 receptor complex and inhibits IRAK1 phosphorylation and kinase activity. It oligomerizes and binds to TLR2 and the TLR4-MD2 complex via its C terminus. It exists as a complex with IRAK1 in unstimulated cells. Upon IL1 signaling, Tollip binds to the activated IL1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. TOLLIP also interacts with TLR2 and TLR4, TOLLIP overexpression inhibits nuclear factor kappa-B (NFKB) activation in response to lipopolysaccharide and IL1B.

Molecular Weight: 30282

NCBI Accession: NP_061882

UniProt: Q9H0E2