ERK2 antibody (Mitogen-Activated Protein Kinase 1) (AA 154-183)

Details for Product anti-MAPK1 Antibody No. ABIN1882240, Supplier: Log in to see
Antigen
  • ATMPK1
  • F14N23.9
  • F14N23_9
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • ATMPK2
  • MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2
  • T30E16.13
  • T30E16_13
  • mitogen-activated protein kinase homolog 2
  • ERK2
  • zERK2
  • MAPK1
  • ERK
  • ERT1
  • MAPK2
  • P42MAPK
  • PRKM1
  • PRKM2
  • p38
  • p40
  • p41
  • p41mapk
  • 9030612K14Rik
  • AA407128
  • AU018647
  • C78273
  • Erk2
  • Prkm1
  • p42mapk
  • erk
  • erk2
  • ert1
  • mapk
  • mpk1
  • xp42
  • mapk2
  • prkm1
  • prkm2
  • Xp42
  • mapk1b
  • mapk1
  • mapk1a
  • mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 2
  • mitogen activated protein kinase 1
  • ATMPK1
  • MPK1
  • MPK2
  • MAPK1
  • mapk1
  • Mapk1
  • PfMAP1
  • PVX_084965
  • Tsp_01601
  • mapk1-b
  • mapk1-a
Alternatives
anti-Human ERK2 antibody for Western Blotting
Epitope
AA 154-183
61
21
16
11
9
8
8
8
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
355
148
120
50
31
19
14
11
10
9
8
7
5
5
2
2
2
2
2
1
1
1
Host
Mouse
171
167
17
8
3
Clonality (Clone)
Monoclonal ()
Conjugate
This ERK2 antibody is un-conjugated
10
9
8
7
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
Application
Western Blotting (WB)
329
157
97
84
82
63
52
26
22
10
5
3
3
2
2
1
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen This Erk2 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from human Erk2.
Clone 814CT5-4-2
Isotype IgG1, kappa
Predicted Reactivity Cow (Bovine),Rat (Rattus),Xenopus laevis
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Alternative Name Erk2 (MAPK1 Antibody Abstract)
Background Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The the MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

Synonyms: MAPK1,ERK2, PRKM1, PRKM2, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 1, ERT1, Mitogen-activated protein kinase 1, Extracellular signal-regulated kinase 2, Mitogen-activated protein kinase 1, MAP kinase isoform p42, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2
NCBI Accession NP_002736, NP_620407
UniProt P28482
Research Area Signaling, Inflammation
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals
Application Notes WB: 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.35 mg/mL
Buffer Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 154-183) antibody (ABIN1882240) MAPK1 Antibody (Center) (ABIN1882240) western blot analysis in MCF-7,HepG2,NCI-H292,H...
Background publications Gevaert, Goethals, Martens, Van Damme, Staes, Thomas, Vandekerckhove: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

Dunham, Shimizu, Roe, Chissoe, Hunt, Collins, Bruskiewich, Beare, Clamp, Smink, Ainscough, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beasley, Bird, Blakey, Bridgeman, Buck, Burgess, Burrill et al.: "The DNA sequence of human chromosome 22. ..." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).

Product cited in: Sgouras, Athanasiou, Beal, Fisher, Blair, Mavrothalassitis: "ERF: an ETS domain protein with strong transcriptional repressor activity, can suppress ets-associated tumorigenesis and is regulated by phosphorylation during cell cycle and mitogenic stimulation." in: The EMBO journal, Vol. 14, Issue 19, pp. 4781-93, 1995 (PubMed).

Owaki, Makar, Boulton, Cobb, Geppert: "Extracellular signal-regulated kinases in T cells: characterization of human ERK1 and ERK2 cDNAs." in: Biochemical and biophysical research communications, Vol. 182, Issue 3, pp. 1416-22, 1992 (PubMed).

Gonzalez, Raden, Rigby, Davis: "Heterogeneous expression of four MAP kinase isoforms in human tissues." in: FEBS letters, Vol. 304, Issue 2-3, pp. 170-8, 1992 (PubMed).

Did you look for something else?