Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

H2AFX antibody (N-Term)

H2AFX Reactivity: Human WB, IHC (p) Host: Mouse Monoclonal 980CT1-5-5 unconjugated
Catalog No. ABIN1882251
  • Target See all H2AFX Antibodies
    H2AFX (H2A Histone Family, Member X (H2AFX))
    Binding Specificity
    • 44
    • 27
    • 18
    • 11
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-30, N-Term
    Reactivity
    • 183
    • 85
    • 60
    • 26
    • 26
    • 20
    • 17
    • 12
    • 9
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Human
    Host
    • 163
    • 20
    • 2
    Mouse
    Clonality
    • 128
    • 57
    Monoclonal
    Conjugate
    • 121
    • 8
    • 6
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This H2AFX antibody is un-conjugated
    Application
    • 146
    • 53
    • 51
    • 43
    • 36
    • 27
    • 25
    • 24
    • 16
    • 12
    • 8
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Predicted Reactivity
    Zf, M
    Purification
    This antibody is purified through a protein G column, followed by dialysis against PBS.
    Immunogen
    This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human H2AFX.
    Clone
    980CT1-5-5
    Isotype
    IgG1
    Top Product
    Discover our top product H2AFX Primary Antibody
  • Application Notes
    WB: 1:1000. IHC-P: 1:25
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Expiry Date
    6 months
  • Stiff, ODriscoll, Rief, Iwabuchi, Löbrich, Jeggo: "ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation." in: Cancer research, Vol. 64, Issue 7, pp. 2390-6, (2004) (PubMed).

    Lukas, Melander, Stucki, Falck, Bekker-Jensen, Goldberg, Lerenthal, Jackson, Bartek, Lukas: "Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention." in: The EMBO journal, Vol. 23, Issue 13, pp. 2674-83, (2004) (PubMed).

    Park, Chan, Park, Oettinger, Kwon: "DNA-PK is activated by nucleosomes and phosphorylates H2AX within the nucleosomes in an acetylation-dependent manner." in: Nucleic acids research, Vol. 31, Issue 23, pp. 6819-27, (2003) (PubMed).

  • Target
    H2AFX (H2A Histone Family, Member X (H2AFX))
    Alternative Name
    H2AFX (H2AFX Products)
    Synonyms
    H2A.X antibody, H2A/X antibody, H2AX antibody, AW228881 antibody, H2ax antibody, Hist5-2ax antibody, gammaH2ax antibody, zgc:56329 antibody, h2a.x antibody, h2a/x antibody, h2ax antibody, RGD1566119 antibody, h2a antibody, h2afx antibody, H2A histone family member X antibody, H2A histone family, member X antibody, H2A histone family member X L homeolog antibody, histone cluster 1, H2ah antibody, histone cluster 2, H2ab S homeolog antibody, histone H2AX antibody, H2AFX antibody, H2afx antibody, h2afx antibody, h2afx.L antibody, HIST1H2AH antibody, hist2h2ab.S antibody, LOC100522201 antibody, LOC100720536 antibody
    Background
    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
    Molecular Weight
    15145
    NCBI Accession
    NP_002096
    UniProt
    P16104
    Pathways
    Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
You are here:
Support