YY1 antibody

Catalog No. ABIN1882292

Product Details anti-YY1 Antibody

Target: YY1 (YY1 Transcription Factor (YY1))

Reactivity: Human, Rat, Zebrafish (Danio rerio), Cynomolgus

Host: Mouse

Clonality: Monoclonal

Conjugate: This YY1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified His-tagged YY1 protein was used to produced this monoclonal antibody.

Clone: 1183CT6-5-23-6

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:1000. WB: 1:1000. IHC-P: 1:25. IHC-P: 1:25. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-YY1 antibody (ABIN1882292)

Fernando, Contreras, Zampini, Rodriguez-Malave, Alberti, Anguiano, Tran, Palanichamy, Gajeton, Ung, Aros, Waters, Casero, Basso, Pigazzi, Rao: "The lncRNA CASC15 regulates SOX4 expression in RUNX1-rearranged acute leukemia." in: Molecular cancer, Vol. 16, Issue 1, pp. 126, (2018) (PubMed).

Burkard, Planyavsky, Kaupe, Breitwieser, Bürckstümmer, Bennett, Superti-Furga, Colinge: "Initial characterization of the human central proteome." in: BMC systems biology, Vol. 5, pp. 17, (2011) (PubMed).

Chen, Cai, Jin, Florens, Swanson, Washburn, Conaway, Conaway: "Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling." in: The Journal of biological chemistry, Vol. 286, Issue 13, pp. 11283-9, (2011) (PubMed).

Kim, Kim: "YY1's longer DNA-binding motifs." in: Genomics, Vol. 93, Issue 2, pp. 152-8, (2009) (PubMed).

Target Details for YY1

Target: YY1 (YY1 Transcription Factor (YY1))

Alternative Name: YY1 (YY1 Products)

Synonyms: yy1-a antibody, FIII antibody, MGC83714 antibody, yy1l antibody, zgc:64207 antibody, YY1 antibody, LOC494431 antibody, xyy1 antibody, delta antibody, nf-e1 antibody, ucrbp antibody, yin-yang-1 antibody, LOC100304682 antibody, ino80s antibody, yy1 antibody, DELTA antibody, INO80S antibody, NF-E1 antibody, UCRBP antibody, YIN-YANG-1 antibody, AW488674 antibody, fb59g10 antibody, wu:fa16g07 antibody, wu:fb59g10 antibody, YY1 transcription factor L homeolog antibody, YY1 transcription factor b antibody, YY1 transcription factor antibody, transcriptional repressor protein YY1 antibody, YY1 transcription factor S homeolog antibody, YY1 transcription factor a antibody, YY2 transcription factor antibody, yy1.L antibody, yy1b antibody, YY1 antibody, yy1 antibody, LOC100304682 antibody, yy1.S antibody, Yy1 antibody, yy1a antibody, YY2 antibody

Background: Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3', some genes have been shown to contain a longer binding motif allowing enhanced binding, the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair, proposed to target the INO80 complex to YY1-responsive elements.

Molecular Weight: 44713

NCBI Accession: NP_003394

UniProt: P25490