SensoLyte® AFC Caspase Profiling Kit

Catalog No. ABIN1882425

Product Details

Target: Caspase (CASP)

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® Caspase Profiling Kit contains a series of AFC-based peptide substrates (Ex/Em=380 nm/500 nm) as fluorogenic indicators for assaying caspase protease activities. The kit contains a well-designed plate in which a series of AFC-based caspase substrates are coated with both positive and negative controls. It provides the best solution for profiling caspases or caspase inhibitors.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Keep Components A and B away from light

Storage: -20 °C

References

Jaiswal, Agrawal, Mallers, Gilman-Sachs, Hirsch, Beaman: "Regulation of apoptosis and innate immune stimuli in inflammation-induced preterm labor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 11, pp. 5702-13, (2013) (PubMed).

Shteingart, Hadar, Cohen, Ravid, Tirosh: "Endoplasmic reticulum stress induces a caspase-dependent N-terminal cleavage of RBX1 protein in B cells." in: The Journal of biological chemistry, Vol. 287, Issue 37, pp. 31223-32, (2012) (PubMed).

Lopez, Kelleher: "Zip6-attenuation promotes epithelial-to-mesenchymal transition in ductal breast tumor (T47D) cells." in: Experimental cell research, Vol. 316, Issue 3, pp. 366-75, (2010) (PubMed).

Target Details

Target: Caspase (CASP)

Alternative Name: Caspase (CASP Products)

Synonyms: CG5370 Kit, DCP-1 Kit, DCP1 Kit, Dcp1 Kit, Dmel\\CG5370 Kit, ccp1 Kit, dcp-1 Kit, dcp1 Kit, l(2)01862 Kit, l(2)02132 Kit, Death caspase-1 Kit, Dcp-1 Kit

Background: Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AFC-based substrates yield blue fluorescence upon protease cleavage. They are widely used to monitor caspase activity.