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Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody

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Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA)
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Pubmed 11 references available
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Quantity 25 μL
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Immunogen The immunogen recognized by this antibody maps to a region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1 (Q16665).
Cross-Reactivity (Details) Monkey (COS-7), rat,(review feedback). Has been shown to detect HIF-1 alpha in both mouse,human tissue by IHC with Citrate buffer antigen retrieval. Based on 100% sequence identity, this antibody is expected to react with: Cavia porcellus, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Grass carp, Northern pike, Atlantic cod, Duckbill platypus,Gansu zokor.
Purification affinity purified
Alternative Name HIF1A / HIF1 alpha (HIF1A Antibody Abstract)
Background Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as aposttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composedof HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated.However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 orPro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degradedin proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIFdegradation and increases transcriptional activity. Therefore, the concentration of HIF-1alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition.HIF hydroxylases provide insight into hypoxic cell responses, which may be used to helpisolate therapeutic targets. Alternate Names: anti-Hypoxia-inducible factor 1 alpha antibody, anti-HIF-1alpha antibody, anti-ARNTinteracting proteinantibody, anti-Hif1a antibody, anti-ARNT interacting protein antibody,anti-HIF1Alpha antibody, anti-Hypoxia inducible factor 1 alpha antibody, anti-Hypoxiainducible factor 1 alpha subunit basic helix antibody.
Gene Symbol: HIF1A
Gene ID 3091, 15251
Application Notes This antibody is useful for Western blot, Immunoprecipitation, FLOW, ELISA, Immunocytochemistry/Immunofluorescence and Immunohistochemistry application. For IHC-P, Tris-EDTA pH 9.0 buffer is recommended for the heat induced epitope retrieval.
Recommended dilutions: ELISA 1:100-1:2000, Flow Cytometry 0.125 µg per 1 million cells in a 150 mcl reaction, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:50-1:200, Immunohistochemistry-Paraffin 1:50-1:200, Immunoprecipitation 2-5 µg/mg lysate, Proximity Ligation Assay 1:200-1:4000, Western Blot 1:2000-1:10000
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer Tris-buffered Saline and 0.1 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN188610) Detection of mouse HIF1-alpha on hypoxia treated MEFs using ABIN151900.
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN188610) anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN188610) Mouse Brain, Neurons 40X
Product cited in: Vangeison, Carr, Federoff et al.: "The good, the bad, and the cell type-specific roles of hypoxia inducible factor-1 alpha in neurons and astrocytes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 8, pp. 1988-93, 2008 (PubMed).

Becker, Rohwer, Funakoshi et al.: "2-methoxyestradiol inhibits hypoxia-inducible factor-1{alpha} and suppresses growth of lesions in a mouse model of endometriosis." in: The American journal of pathology, Vol. 172, Issue 2, pp. 534-44, 2008 (PubMed).

May, Gilon, Djonov et al.: "Transgenic system for conditional induction and rescue of chronic myocardial hibernation provides insights into genomic programs of hibernation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 1, pp. 282-7, 2008 (PubMed).

Takeda, Aguila, Parikh et al.: "Regulation of adult erythropoiesis by prolyl hydroxylase domain proteins." in: Blood, Vol. 111, Issue 6, pp. 3229-35, 2008 (PubMed).

Glassford, Yue, Sheikh et al.: "HIF-1 regulates hypoxia- and insulin-induced expression of apelin in adipocytes." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 6, pp. E1590-6, 2007 (PubMed).

Palmer, Doctor, Chhabra et al.: "S-nitrosothiols signal hypoxia-mimetic vascular pathology." in: The Journal of clinical investigation, Vol. 117, Issue 9, pp. 2592-601, 2007 (PubMed).

Chen, Pistollato, Hoeppner et al.: "Oxygen tension regulates survival and fate of mouse central nervous system precursors at multiple levels." in: Stem cells (Dayton, Ohio), Vol. 25, Issue 9, pp. 2291-301, 2007 (PubMed).

Nagano, Yamashita, Hamada et al.: "Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood." in: Blood, Vol. 110, Issue 1, pp. 151-60, 2007 (PubMed).

Takeda, Ho, Takeda et al.: "Placental but not heart defects are associated with elevated hypoxia-inducible factor alpha levels in mice lacking prolyl hydroxylase domain protein 2." in: Molecular and cellular biology, Vol. 26, Issue 22, pp. 8336-46, 2006 (PubMed).

Litz, Krystal: "Imatinib inhibits c-Kit-induced hypoxia-inducible factor-1alpha activity and vascular endothelial growth factor expression in small cell lung cancer cells." in: Molecular cancer therapeutics, Vol. 5, Issue 6, pp. 1415-22, 2006 (PubMed).

Hsu, Adereth, Kose et al.: "Endocytic function of von Hippel-Lindau tumor suppressor protein regulates surface localization of fibroblast growth factor receptor 1 and cell motility." in: The Journal of biological chemistry, Vol. 281, Issue 17, pp. 12069-80, 2006 (PubMed).

Catalog No. ABIN188610

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