Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Antigen: Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A)

Synonyms: HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A, HIF-1a, HIF1a, hif-1a, hif1a

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN188610

Additional Information

Alternative name: HIF1A / HIF1 alpha

Gene ID: 3091

Immunogen: A synthetic peptide mapping to a region between residues 775 and the C-terminus(residue 826) of human hypoxia-inducible factor 1, alpha subunit using the numberinggiven in Swiss-Prot entry Q16665 (GeneID 3091).

Cross-Reactivity: Human, Mouse (Murine)

Description: Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as aposttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composedof HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated.However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 orPro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degradedin proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIFdegradation and increases transcriptional activity. Therefore, the concentration of HIF-1alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition.HIF hydroxylases provide insight into hypoxic cell responses, which may be used to helpisolate therapeutic targets. Alternate Names: anti-Hypoxia-inducible factor 1 alpha antibody, anti-HIF-1alpha antibody, anti-ARNTinteracting protein antibody, anti-Hif1a antibody, anti-ARNT interacting protein antibody,anti-HIF1Alpha antibody, anti-Hypoxia inducible factor 1 alpha antibody, anti-Hypoxiainducible factor 1 alpha subunit basic helix antibody.

Specificity: Species Reactivity: Human, monkey, fish and Mouse HIF-1 alpha in Western blot analysis. Prelimary resultswith Mouse tissue have been positive for immunohistochemistry*. Used at 1:100 onMouse eye with high pH retrieval.

Application Details

Application Notes: Suggested working dilutions: flow cytometry / FACS analysis 0.125 mcg per 1 million cells,immunohistochemistry 1:100,Western Blot 1:500-1:2500,Immunohistochemistry-Paraffin 1:100

Concentration: 0.2 mg/ml

Purification: Aff - Purified

Buffer: BSA in TBS. Preservative: 0.09% Sodium Azide.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

Western Blot: anti-HIF-1 alpha Antibody [ABIN151900SS] - Detection of mouse HIF1-alpha unsing ABIN151900.

Western Blot: anti-HIF-1 alpha Antibody [ABIN151900SS] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (hypoxic). Lane 2: Untreated Cos-7 nuclear extract (normoxic).

Immunohistochemistry: anti-HIF-1 alpha Antibody [ABIN151900SS] - Mouse Brain, Neurons 40X

Publications

Hsu, Adereth, Kose et al.: "Endocytic function of von Hippel-Lindau tumor suppressor protein regulates surface localization of fibroblast growth factor receptor 1 and cell motility." in: The Journal of biological chemistry, Vol. 281, Issue 17, pp. 12069-80, 2006 (PubMed).

Litz, Krystal: "Imatinib inhibits c-Kit-induced hypoxia-inducible factor-1alpha activity and vascular endothelial growth factor expression in small cell lung cancer cells." in: Molecular cancer therapeutics, Vol. 5, Issue 6, pp. 1415-22, 2006 (PubMed).

Nagano, Yamashita, Hamada et al.: "Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood." in: Blood, Vol. 110, Issue 1, pp. 151-60, 2007 (PubMed).

Chen, Pistollato, Hoeppner et al.: "Oxygen tension regulates survival and fate of mouse central nervous system precursors at multiple levels." in: Stem cells (Dayton, Ohio), Vol. 25, Issue 9, pp. 2291-301, 2007 (PubMed).

Tam, Wong, Tam et al.: "The effect of acute intermittent hypercapnic hypoxia treatment on IL-6, TNF-alpha, and CRP levels in piglets." in: Sleep, Vol. 30, Issue 6, pp. 723-7, 2007 (PubMed).

Palmer, Doctor, Chhabra et al.: "S-nitrosothiols signal hypoxia-mimetic vascular pathology." in: The Journal of clinical investigation, Vol. 117, Issue 9, pp. 2592-601, 2007 (PubMed).

Glassford, Yue, Sheikh et al.: "HIF-1 regulates hypoxia- and insulin-induced expression of apelin in adipocytes." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 6, pp. E1590-6, 2007 (PubMed).

Takeda, Aguila, Parikh et al.: "Regulation of adult erythropoiesis by prolyl hydroxylase domain proteins." in: Blood, Vol. 111, Issue 6, pp. 3229-35, 2008 (PubMed).

May, Gilon, Djonov et al.: "Transgenic system for conditional induction and rescue of chronic myocardial hibernation provides insights into genomic programs of hibernation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 1, pp. 282-7, 2008 (PubMed).

Becker, Rohwer, Funakoshi et al.: "2-methoxyestradiol inhibits hypoxia-inducible factor-1{alpha} and suppresses growth of lesions in a mouse model of endometriosis." in: The American journal of pathology, Vol. 172, Issue 2, pp. 534-44, 2008 (PubMed).

Vangeison, Carr, Federoff et al.: "The good, the bad, and the cell type-specific roles of hypoxia inducible factor-1 alpha in neurons and astrocytes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 8, pp. 1988-93, 2008 (PubMed).