HCST antibody (Hematopoietic Cell Signal Transducer) (C-Term)

Details for Product anti-HCST Antibody No. ABIN188679, Supplier: Log in to see
Antigen
  • HCST
  • DAP10
  • KAP10
  • PIK3AP
  • Dap10
  • hematopoietic cell signal transducer
  • HCST
  • hcst
  • Hcst
Epitope
C-Term, Isoform 1, Isoform 2
12
2
1
Reactivity
Human
30
1
1
1
Host
Goat
15
15
Clonality
Polyclonal
Conjugate
This HCST antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
15
13
12
3
1
Options
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Immunogen Peptide with sequence C-RSPAQEDGKVYIN corresponding to C-Terminus according to NP_001007470.1.
Specificity This antibody is expected to recognise isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking residue E81 (NP_001007470.1).
Purification Immunogen affinity purified
Alternative Name HCST (HCST Antibody Abstract)
Background Gene Symbol: HCST
Gene ID 10870
NCBI Accession NP_055081
Research Area Immunology
Application Notes Western Blot 0.2 - 0.6 μg/mL, Peptide ELISA Detection limit 1:128000WB: Approx. 40 kDa band observed in human spleen lysates is reported in scientific literature (PMID: 10426994). An additional band of unknown identity was also consistently observed at 100 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL Tris ( pH 7.3) and 0.5 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Hematopoietic Cell Signal Transducer (HCST) (C-Term), (Isoform 1), (Isoform 2) antibody (ABIN188679) ABIN188679 (0.2?g/ml) staining of Human Spleen lysate (35?g protein in RIPA buffer). ...
Western Blotting (WB) image for anti-Hematopoietic Cell Signal Transducer (HCST) (C-Term), (Isoform 1), (Isoform 2) antibody (ABIN188679) Western Blot: HCST Antibody [ABIN1886793] - (0.2ug/ml) staining of human spleen lysat...
Product cited in: Upshaw, Arneson, Schoon, Dick, Billadeau, Leibson: "NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells." in: Nature immunology, Vol. 7, Issue 5, pp. 524-32, 2006 (PubMed).

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