Forkhead Box P2 (FOXP2) antibody

Antigen: Forkhead Box P2 (FOXP2)

Synonyms: SPCH1, CAGH44, TNRC10, DKFZp686H1726, CAG-16, D0Kist7, AI449000, 2810043D05Rik, FOXP2, RGD1559697, spch1, cagh44, tnrc10, xlFoxP2, foxP2, foxP, Foxp2, foxp1c

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN188687

Additional Information

Alternative name: FOXP2

Gene ID: 93986, 500037

NCBI Acession: NP_0553061

Immunogen: Synthetic peptide, C-DEVEYQKRRSQKIT, representing the internal region of the humanprotein according to NP_055306.1, NP_683696.1, NP_683697.1, NP_683698.1. Reference Sequence: NP_055306.1, NP_683696.1, NP_683697.1, NP_683698.1.

Cross-Reactivity: Human, Rat (Rattus)

Isotype: IgG

Description: This gene encodes an evolutionarily conserved transcription factor expressed in fetaland adult brain. This transcription factor is a member of the forkhead/winged-helix(FOX) family of transcription factors, and contains a FOX DNA-binding domain and alarge polyglutamine tract. Members of the FOX family of transcription factors areregulators of embryogenesis. The product of this gene is thought to be required forproper development of speech and language regions of the brain during embryogenesis.Although a point mutation in this gene has been associated with the KE pedigreesegregating developmental verbal dyspraxia, no association between mutations in thisgene and another speech disorder, autism, has been found. Four alternative transcriptsencoding three different isoforms have been identified. Alternate Names: anti-FOXP2 antibody, anti-forkhead box P2antibody, anti-CAGH44 antibody,anti-DKFZp686H1726 antibody, anti-SPCH1 antibody, anti-TNRC10antibody, anti-CAGrepeat protein 44 antibody, anti-forkhead/winged-helix transcription factor antibody,anti-speech and language disorder 1 antibody, anti-trinucleotide repeat containing 10antibody.

Specificity: Species Reactivity: Reacts with Human and rat. Expected from sequence similarity: Mouse and Canine.

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 85kDaband observed in Human Brain (Cerebellum and Frontal Cortex) lysates and in RatBrain lysates (calculated MW of 82.6kDa according to NP_683696.1). Recommendedconcentration: 0.3-1ug/ml.An additional band of unknown identity was also consistently observed at 28-30kDa.This band was successfully blocked by incubation with the immunising peptide. Wewould appreciate any feedback from people in the field - have any such results beenreported with other antibodies/lysates? Have any further splice variants/modified formsbeen reported?

Concentration: 0.5 mg/ml

Purification: Aff - Purified

Buffer: Tris-saline [ pH7.3] with 0.5% BSA. Preservative: 0.02% sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

ABIN188687 (0.3?g/ml) staining of Human Brain (Cerebellum) lysate (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Lai, Fisher, Hurst et al.: "A forkhead-domain gene is mutated in a severe speech and language disorder." in: Nature, Vol. 413, Issue 6855, pp. 519-23, 2001 (PubMed).

Zhou, Zhong, Minoo et al.: "Foxp2 inhibits Nkx2.1-mediated transcription of SP-C via interactions with the Nkx2.1 homeodomain." in: American journal of respiratory cell and molecular biology, Vol. 38, Issue 6, pp. 750-8, 2008 (PubMed).

Prasad, Wang, Gray et al.: "A differential developmental pattern of spinal interneuron apoptosis during synaptogenesis: insights from genetic analyses of the protocadherin-gamma gene cluster." in: Development (Cambridge, England), Vol. 135, Issue 24, pp. 4153-64, 2008 (PubMed).