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HVEM ELISA Kit

TNFRSF14 Reactivity: Human AA 39-202 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889390
  • Target See all HVEM (TNFRSF14) ELISA Kits
    HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
    Binding Specificity
    AA 39-202
    Reactivity
    • 4
    • 3
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6-1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFRSF14/HVEM
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: L39-V202
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <1pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: L39-V202
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 3 TNFR-Cys repeats.

    Tissue Specificity: Widely expressed, with the highest expression in lung, spleen and thymus.

    Plate
    Pre-coated
    Protocol
    human TNFRSF14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF14 has been precoated onto 96-well plates. Standards(NSO, L39-V202) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF14 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human TNFRSF14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNFRSF14 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 67, Standard deviation: 2.68, CV(%): 4
    • Sample 2: n=16, Mean(pg/ml): 284, Standard deviation: 9.94, CV(%): 3.5
    • Sample 3: n=16, Mean(pg/ml): 626, Standard deviation: 32.6, CV(%): 5.2,
    • Sample 1: n=24, Mean(pg/ml): 87, Standard deviation: 4.61, CV(%): 5.3
    • Sample 2: n=24, Mean(pg/ml): 324, Standard deviation: 15.55, CV(%): 4.8
    • Sample 3: n=24, Mean(pg/ml): 682, Standard deviation: 41.6, CV(%): 6.1
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all HVEM (TNFRSF14) ELISA Kits
    HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
    Alternative Name
    TNFRSF14 (TNFRSF14 Products)
    Synonyms
    ATAR ELISA Kit, CD270 ELISA Kit, HVEA ELISA Kit, HVEM ELISA Kit, LIGHTR ELISA Kit, TR2 ELISA Kit, Atar ELISA Kit, HveA ELISA Kit, Hvem ELISA Kit, Tnfrs14 ELISA Kit, TNF receptor superfamily member 14 ELISA Kit, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) ELISA Kit, Tnfrsf14 ELISA Kit, TNFRSF14 ELISA Kit
    Target Type
    Viral Protein
    Background

    Protein Function: Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells. .

    Background: Tumor necrosis factor receptor superfamily member 14(TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which are form a bidirectional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.

    Synonyms: Tumor necrosis factor receptor superfamily member 14,Herpes virus entry mediator A,Herpesvirus entry mediator A,HveA,Tumor necrosis factor receptor-like 2,TR2,CD270,TNFRSF14,HVEA, HVEM,UNQ329/PRO509,

    Full Gene Name: Tumor necrosis factor receptor superfamily member 14

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    Gene ID
    8764
    UniProt
    Q92956
    Pathways
    Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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