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Cathepsin E ELISA Kit

CTSE Reactivity: Human AA 18-396 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889446
  • Target See all Cathepsin E (CTSE) ELISA Kits
    Cathepsin E (CTSE)
    Binding Specificity
    AA 18-396
    Reactivity
    • 4
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10.000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Cathepsin E
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: Q18-P396
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: Q18-P396
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B- lymphocytes. .

    Plate
    Pre-coated
    Protocol
    human Cathepsin E ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin E has been precoated onto 96-well plates. Standards(NSO, Q18-P396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin E is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin E amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Cathepsin E standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Cathepsin E standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.95, Standard deviation: 0.099, CV(%): 5.1
    • Sample 2: n=16, Mean(ng/ml): 4.67, Standard deviation: 0.201, CV(%): 4.3
    • Sample 3: n=16, Mean(ng/ml): 6.05, Standard deviation: 0.218, CV(%): 3.6,
    • Sample 1: n=24, Mean(ng/ml): 2.34, Standard deviation: 0.149, CV(%): 6.4
    • Sample 2: n=24, Mean(ng/ml): 4.83, Standard deviation: 0.28, CV(%): 5.8
    • Sample 3: n=24, Mean(ng/ml): 6.58, Standard deviation: 0.349, CV(%): 5.3
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all Cathepsin E (CTSE) ELISA Kits
    Cathepsin E (CTSE)
    Alternative Name
    CTSE (CTSE Products)
    Synonyms
    CATE ELISA Kit, A430072O03Rik ELISA Kit, C920004C08Rik ELISA Kit, CE ELISA Kit, CatE ELISA Kit, CEA ELISA Kit, CEB ELISA Kit, Ctsea ELISA Kit, CTSE ELISA Kit, ce1 ELISA Kit, ce2 ELISA Kit, cate ELISA Kit, CE1 ELISA Kit, ce1-A ELISA Kit, ctse ELISA Kit, CE2 ELISA Kit, ce2-A ELISA Kit, cathepsin E ELISA Kit, cathepsin E L homeolog ELISA Kit, cathepsin E S homeolog ELISA Kit, CTSE ELISA Kit, Ctse ELISA Kit, TP03_0056 ELISA Kit, Smp_184930 ELISA Kit, ctse ELISA Kit, ctse.L ELISA Kit, ctse.S ELISA Kit
    Background

    Protein Function: May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain. .

    Background: Cathepsin E is a protein that in humans is encoded by the CTSE gene. It is mapped to 1q32.1. The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. Cathepsin E is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen, and it also has an important role in immune responses.

    Synonyms: Cathepsin E,3.4.23.34,Cathepsin E form I,Cathepsin E form II,CTSE,

    Full Gene Name: Cathepsin E

    Cellular Localisation: Endosome . The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome.
    Gene ID
    1510
    UniProt
    P14091
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