TAR DNA Binding Protein (TARDBP) antibody

Antigen: TAR DNA Binding Protein (TARDBP)

Synonyms: ALS10, TDP-43, Tdp43, C85084, 1190002A23Rik, fb77f02, fc52g10, wu:fb77f02, wu:fc52g10, tardbp, MGC53141, TARDBP, TDP43, DKFZp459I2127, MGC129120

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN188986

Additional Information

Alternative name: TARDBP

Gene ID: 23435, 230908

UniProt: Q13148

Immunogen: A synthetic peptide to a C-terminal region [within residues 350-414] of the humanTARDBP protein. [Swiss-Prot# Q13148].

Cross-Reactivity: Human

Description: The HIV1 virus gives rise to acquired immunodeficiency syndrome (AIDS). HIV1contains an RNA genome which produces chromosomally integrated DNA duringcellular replication. Gene activation of HIV1 by the transactivator Tat requires an RNAregulatory element TAR which is located downstream of the transcription initiation site.TARDBP is a transcriptional repressor that binds to the chromosomally integrated TARDNA, preventing HIV1 expression. Additionally, this protein regulates alternative splicingof the CTFR gene, a similar pseudogene on chromosoome 20. TARDBP has also been recently been shown to abnormally accumulate in thepost-mortem brain tissue of individuals diagnosed with ALS (Amyotrophic LateralSclerosis) and FTD (Frontal Temporal Dementia). This long-awaited conncectionbetween these two diseases provides an explanation for the observed clinical overlap. Alternate Names: anti-TARD binding protein antibody, anti-TAR DNA-binding protein 43 antibody,anti-TDP-43 antibody, anti-TDP43 antibody.

Specificity: Species Reactivity: Reacts with human, primate, mouse, chicken, Xenopus, and Zebrafish. Localization: Nuclear.

Application Details

Application Notes: Suggested working dilutions: Western Blot 0.5 to 2.0 ug/ ml

Concentration: 1 mg/ml

Purification: Aff - Purified

Buffer: Tris-glycine, 150mM NaCl. Preservative: 0.05% sodium azide.

Storage: Store at 4C. Do not freeze.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors, DNA/RNA, Hormones, Cytokines

Restrictions: For Research Use only

Images

Detection of TARDBP in HeLa WCE using ABIN188986.

Publications

Neumann, Sampathu, Kwong et al.: "Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis." in: Science (New York, N.Y.), Vol. 314, Issue 5796, pp. 130-3, 2006 (PubMed).

Mackenzie, Bigio, Ince et al.: "Pathological TDP-43 distinguishes sporadic amyotrophic lateral sclerosis from amyotrophic lateral sclerosis with SOD1 mutations." in: Annals of neurology, Vol. 61, Issue 5, pp. 427-34, 2007 (PubMed).

Geser, Brandmeir, Kwong et al.: "Evidence of multisystem disorder in whole-brain map of pathological TDP-43 in amyotrophic lateral sclerosis." in: Archives of neurology, Vol. 65, Issue 5, pp. 636-41, 2008 (PubMed).