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TAR DNA Binding Protein (TARDBP) (AA 350-414), (C-Term) antibody

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Synonyms TARDBP, TDP43, DKFZp459I2127, tardbp, ALS10, TDP-43, 1190002A23Rik, C85084, Tdp43, wu:fb77f02, wu:fc52g10
C-Term, AA 350-414
(29), (22), (16), (15), (13), (12), (12), (10), (7), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1)
Chicken, Human, Monkey, Mouse (Murine), Xenopus laevis, Zebrafish (Danio rerio)
(168), (70), (55), (10), (8), (8), (7), (6), (5), (4), (3), (2), (1), (1)
(124), (36), (15), (1)
(7), (6), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
(155), (76), (54), (42), (37), (22), (12), (11), (7), (5), (4), (3), (2), (1)
Pubmed 3 references available
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Quantity 25 μL
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Immunogen A synthetic peptide to a C-terminal region [within residues 350-414] of the human TARDBP protein.
Purification affinity purified
Alternative Name TARDBP (TARDBP Antibody Abstract)
Background The HIV1 virus gives rise to acquired immunodeficiency syndrome (AIDS). HIV1contains an RNA genome which produces chromosomally integrated DNA duringcellular replication. Gene activation of HIV1 by the transactivator Tat requires an RNAregulatory element TAR which is located downstream of the transcription initiation site.TARDBP is a transcriptional repressor that binds to the chromosomally integrated TARDNA, preventing HIV1 expression. Additionally, this protein regulates alternative splicingof the CTFR gene, a similar pseudogene on chromosoome 20. TARDBP has also been recently been shown to abnormally accumulate in thepost-mortem brain tissue of individuals diagnosed with ALS (Amyotrophic LateralSclerosis) and FTD (Frontal Temporal Dementia). This long-awaited conncectionbetween these two diseases provides an explanation for the observed clinical overlap. Alternate Names: anti-TARD binding protein antibody, anti-TAR DNA-binding protein 43 antibody,anti-TDP-43 antibody, anti-TDP43 antibody.
Gene Symbol: TARDBP
Gene ID 23435, 230908
UniProt Q13148
Research Area Chromatin and Nuclear Signaling, Transcription Factors, DNA/RNA, Hormones, Cytokines
Application Notes This TARDBP antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis. In Western blot a band is seen at ~45 kDa, representing the human TARDBP protein. In ICC/IF a nuclear signal is present in MCF-7 cells.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunohistochemistry, Western Blot 0.5-2 µg/mL
Protocol Protocol specific for TARDBP Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % Bis-Tris) on samples to be analyzed, loading 20 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS, overnight at 4 degrees Celcius.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-TARDBP primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-TAR DNA Binding Protein (TARDBP) (AA 350-414), (C-Term) antibody (ABIN188988) Detection of TARDBP in HeLa WCE using ABIN188986.
Product cited in: Geser, Brandmeir, Kwong et al.: "Evidence of multisystem disorder in whole-brain map of pathological TDP-43 in amyotrophic lateral sclerosis." in: Archives of neurology, Vol. 65, Issue 5, pp. 636-41, 2008 (PubMed).

Mackenzie, Bigio, Ince et al.: "Pathological TDP-43 distinguishes sporadic amyotrophic lateral sclerosis from amyotrophic lateral sclerosis with SOD1 mutations." in: Annals of neurology, Vol. 61, Issue 5, pp. 427-34, 2007 (PubMed).

Neumann, Sampathu, Kwong et al.: "Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis." in: Science (New York, N.Y.), Vol. 314, Issue 5796, pp. 130-3, 2006 (PubMed).

Catalog No. ABIN188988
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