anti-beta Actin antibody (Actin, beta) (C-Term)

Details for Product anti-ACTB Antibody No. ABIN188998
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Epitope
C-Term
(136), (37), (14), (14), (12), (10), (7), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(484), (205), (189), (54), (50), (42), (38), (35), (28), (24), (22), (15), (13), (12), (11), (10), (8), (7), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(300), (198), (22), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
This beta Actin antibody is un-conjugated
(29), (21), (15), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(463), (219), (140), (95), (93), (55), (44), (29), (20), (11), (11), (10), (9), (9), (3), (3), (3), (2), (2), (2), (1), (1)
Pubmed 3 references available
Quantity 0.1 mL
Shipping to United States ( )
Availability Please contact our customer support.
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Immunogen A synthetic peptide corresponding to residues near the C-terminus of human Beta Actin (Cytoplasmic Actin) was used as an immunogen.
Clone EP1123Y
Isotype IgG
Purification Tissue culture supernatant
Alternative Name Actin beta / ACTB (ACTB Antibody Abstract)
Background Cytoplasmic (. Alternate Names: anti-ACTB antibody, anti-Actin cytoplasmic 1 antibody, anti-beta cytoskeletal actinantibody, anti-PS1TP5 binding protein 1 antibody, anti-PS1TP5BP1 antibody.
Gene Symbol: ACTB
Gene ID 60, 11461, 81822
Application Notes Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:50, Immunohistochemistry 1:50, Immunohistochemistry-Paraffin 1:50, Western Blot 1:1000-10000
Protocol Protocol specific for Beta Actin Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Actin, beta (ACTB) (C-Term) antibody Western blot analysis on 1) T47D and 2) HeLa cell lysate using anti- Beta Actin (Cyto...
anti-Actin, beta (ACTB) (C-Term) antibody (2) Immunohistochemical analysis of paraffin-embedded human muscle using anti-Beta Actin ...
General Kumar, McClain, Young et al.: "Cholesterol transporter ATP-binding cassette A1 (ABCA1) is elevated in prion disease and affects PrPC and PrPSc concentrations in cultured cells." in: The Journal of general virology, Vol. 89, Issue Pt 6, pp. 1525-32, 2008 (PubMed).

Sasaki, Ishii, Jeyasuria et al.: "Complex role of the mitochondrial targeting signal in the function of steroidogenic acute regulatory protein revealed by bacterial artificial chromosome transgenesis in vivo." in: Molecular endocrinology (Baltimore, Md.), Vol. 22, Issue 4, pp. 951-64, 2008 (PubMed).

Herman: "Actin isoforms." in: Current opinion in cell biology, Vol. 5, Issue 1, pp. 48-55, 1993 (PubMed).

Validation Images
Catalog No. ABIN188998
471.90 $
Plus shipping costs $45.00
Add to Basket

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)
Did you look for something else?