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Activating Transcription Factor 2 (ATF2) antibody

Antigen

Activating Transcription Factor 2 (ATF2)

Synonyms HB16, CREB2, TREB7, CRE-BP1, MGC111558, ATF2, MGC142504, CHN1, CG12850, CG3749, dATF-2, DmelCG30420, CG30420
Clonality Monoclonal (E242)
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human

Application
Alternatives Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Catalog no. ABIN189035
Quantity 0.1 ml
Price Product not available in this region.
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Additional Information

Alternative name ATF2
Gene ID 1386, 11909, 81647
Swiss-Prot P15336
Isotype IgG  (Matching secondary antibodies)
Clone E242
Description ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds thecAMP response element (CRE) and AP-1 response element and is abundantlyexpressed in the brain. Various forms of cellular stress, including genotoxic agents,inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2(2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 byphosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in theloss of stress-induced transcription by ATF-2 (2-4). Alternate Names: anti-Activating Transcription Factor 2 antibody, anti-cAMP Response Element BindingProtein 2 antibody, anti-CRE BP1 antibody, anti-CREB 2 antibody, anti-CREB2antibody, anti-CREBP1 antibody, anti-HB 16 antibody, anti-HB16 antibody,anti-MGC111558 antibody, anti-mXBP antibody, anti-TREB 7 antibody, anti-TREB7antibody.
Specificity A synthetic peptide corresponding to residues N-terminus of human ATF-2 was used asimmunogen. The antibody does not cross-react with other ATF family members. Species Reactivity: Cross reacts with Human. Does not react with Mouse or Rat.

Application Details

Application Notes Suggested working dilutions: immunohistochemistry 1:100,immunoprecipitation 1:50,Western Blot 1:10000,Immunocytochemistry 1:250 to 1:500
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. Preservative: 0.01% sodium azide.
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Research Area Immunology, Innate Immunity, Transcription Factors
Restrictions For Research Use only

Alternatives

Alternatives for antigen "Activating Transcription Factor 2 (ATF2)", type "Antibodies"
Hosts Rabbit (143), Mouse (16), Goat (4), Sheep (3)
Reactivities Human (163), Mouse (Murine) (112), Rat (Rattus) (99), Cow (Bovine) (1), Dog (Canine) (1), Horse (Equine) (1)
Applications Western Blotting (WB) (105), Immunohistochemistry (IHC) (63), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (47), Immunofluorescence (IF) (36), Immunoprecipitation (IP) (32), Enzyme Immunoassay (EIA) (30), ELISA (24), Flow Cytometry (FACS) (14), Dot Blot (Dot) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1)
Conjugates Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pThr69/51 (6), Ab-112 or 94 (5), Ab-62 or 44 (5), Ab-69 or 51 (5), Ab-71 or 53 (5), Ab-73 or 55 (5), pSer112 or 94 (5), pSer62 or 44 (5), pSer62/44 (5), pThr69 or 51 (5), pThr71 or 53 (5), pThr73 or 55 (5), pSer112/94 (4), pThr71/53 (4), pThr73/55 (3), S44 (2), S94 (2), PSer340/322 (1), S322 (1), pSer44 (1), pSer472 (1), pSer94 (1), pThr51 (1), pThr69/51 + pThr71/53 (1), pThr71 (1), pThr79/51 (1)