Cytohesin 3 (CYTH3) antibody

Antigen: Cytohesin 3 (CYTH3)

Synonyms: GRP1, ARNO3, PSCD3, CLM3, Grp1, Pscd3, AI648983, KIAA4241, Sec7, Sec7C, MGC124579, CYTH3, pscd3, MGC145373

Clonality: Monoclonal (EP394Y)

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN189382

Additional Information

Alternative name: Cytohesin 3

Gene ID: 9265, 56149, 192254

UniProt: O43739

Immunogen: A synthetic peptide corresponding to residues near the SEC7 domain of GRP1 was used as immunogen.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG

Clone: EP394Y

Description: GRP1 (general receptor for 3-phosphoinositides) is composed of an N-terminal heptadrepeat, an Arf exchange domain with homology to the yeast Sec7 protein, a PH domain,and a C-terminal polybasic region (1). The PH domain of GRP1 binds PtdIns(3, 4, 5)P3with high affinity, but not PtdIns(3, 4)P2 or PtdIns(3)P, potentially making it a target forPI-3-kinase. Furthermore, GRP1 acts as a nucleotide exchange factor for the small GTPproteins of the ARF family (2). While GRP1 catalyses guanine-nucleotide exchangespecifically on ARF5, other GRP1 homologues (ARNO and cytohesin-1) will onlyfunction on ARF6 and ARF3 respectively (3). Alternate Names: anti-GRASP antibody, anti-GRP1 associated scaffold protein antibody.

Specificity: A synthetic peptide corresponding to residues near the SEC7 domain of GRP1 wasused as immunogen. Species Reactivity: Cross reacts with Human, Mouse and Rat.

Application Details

Application Notes: Suggested working dilutions: Western Blot 1:500

Purification: Aff - Purified

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. Preservative: 0.01% sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Signaling

Restrictions: For Research Use only

Images

Western blot analysis on Hunan brain lysate using anti-GRP1 RabMAb (cat. # 110-57015), dilution 1:500.

Publications

Klarlund, Guilherme, Holik et al.: "Signaling by phosphoinositide-3,4,5-trisphosphate through proteins containing pleckstrin and Sec7 homology domains." in: Science (New York, N.Y.), Vol. 275, Issue 5308, pp. 1927-30, 1997 (PubMed).

Klarlund, Rameh, Cantley et al.: "Regulation of GRP1-catalyzed ADP ribosylation factor guanine nucleotide exchange by phosphatidylinositol 3,4,5-trisphosphate." in: The Journal of biological chemistry, Vol. 273, Issue 4, pp. 1859-62, 1998 (PubMed).