Cytohesin 3 (CYTH3) antibody

Details for Product No. ABIN189382
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Antigen
Reactivity
Human
(35), (11), (9), (1)
Host
Rabbit
(26), (10)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(34), (24), (9), (5), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 0.1 mL
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Availability Discontinued
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Catalog No. ABIN189382
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Immunogen A synthetic peptide corresponding to residues near the MH2 domain of human Smad2 was used as immunogen.
Clone EP394Y
Isotype IgG
Purification IgG purified
Alternative Name Cytohesin 3
Background GRP1 (general receptor for 3-phosphoinositides) is composed of an N-terminal heptadrepeat, an Arf exchange domain with homology to the yeast Sec7 protein, a PH domain,and a C-terminal polybasic region (1). The PH domain of GRP1 binds PtdIns(3, 4, 5)P3with high affinity, but not PtdIns(3, 4)P2 or PtdIns(3)P, potentially making it a target forPI-3-kinase. Furthermore, GRP1 acts as a nucleotide exchange factor for the small GTPproteins of the ARF family (2). While GRP1 catalyses guanine-nucleotide exchangespecifically on ARF5, other GRP1 homologues (ARNO and cytohesin-1) will onlyfunction on ARF6 and ARF3 respectively (3). Alternate Names: anti-GRASP antibody, anti-GRP1 associated scaffold protein antibody.
Gene Symbol: CYTH3
Gene ID 9265, 56149, 192254
Research Area Signaling
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Flow Cytometry. In Western blot this antibody detects a band at approximately 58kDa.
Recommended dilutions: Flow Cytometry 1:20, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry, Immunohistochemistry-Paraffin 1:50, Western Blot 1:1000-10000
Restrictions For Research Use only
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Cytohesin 3 (CYTH3) antibody Western blot analysis on Hunan brain lysate using anti-GRP1 RabMAb (cat. # 110-57015), dilution 1:500.
General Klarlund, Guilherme, Holik et al.: "Signaling by phosphoinositide-3,4,5-trisphosphate through proteins containing pleckstrin and Sec7 homology domains." in: Science (New York, N.Y.), Vol. 275, Issue 5308, pp. 1927-30, 1997 (PubMed).

Klarlund, Rameh, Cantley et al.: "Regulation of GRP1-catalyzed ADP ribosylation factor guanine nucleotide exchange by phosphatidylinositol 3,4,5-trisphosphate." in: The Journal of biological chemistry, Vol. 273, Issue 4, pp. 1859-62, 1998 (PubMed).

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