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TBPL1 antibody (TBP-Like 1)

Details for Product anti-TBPL1 Antibody No. ABIN189464, Supplier: Log in to see
Antigen
  • 4732475G08
  • AW011832
  • AW491032
  • D18347
  • MGC:8389
  • MGC:9620
  • STD
  • STUD
  • TLF
  • tlf
  • Tlp
  • TLP
  • tlp
  • trf
  • TRF2
  • trf2
  • TRP
  • trp
  • zgc:92340
Reactivity
Chinese Hamster, Human, Mouse (Murine), Primate, Rat (Rattus)
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62
51
8
6
6
5
3
1
1
1
1
Host
Rabbit
47
47
17
Clonality
Polyclonal
Conjugate
This TBPL1 antibody is un-conjugated
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5
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4
4
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3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
91
46
41
32
31
29
29
25
20
18
12
2
1
1
Supplier
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Available images

Immunogen Baculovirus purified TRF2 protein.
Purification Immunogen affinity purified
Alternative Name TRF-2 (TBPL1 Antibody Abstract)
Background Gene Symbol: TERF2
Molecular Weight Theoretical MW: 56 kDa
Application Notes Western Blot 1:2000-1:5000, Simple Western 1:25, Chromatin Immunoprecipitation 1:10-1:500, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation, Immunohistochemistry-Paraffin 1:200
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot Protocol Specific for NB110-57130
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunocytochemistry Protocol Specific for NB110-57130 Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-TBPL1 antibody (TBP-Like 1) (ABIN189464) Detection of TRF2 in HeLa nuclear extract using ABIN189464.
Simple Western (SimWes) image for anti-TBPL1 antibody (TBP-Like 1) (ABIN189464) Simple Western: TRF-2 Antibody [ABIN1894640] - Simple Western lane view shows a speci...
Western Blotting (WB) image for anti-TBPL1 antibody (TBP-Like 1) (ABIN189464) Western Blot: TRF-2 Antibody [ABIN1894640] - Analysis of HeLa whole cell lysate (A), ...
Immunoprecipitation (IP) image for anti-TBPL1 antibody (TBP-Like 1) (ABIN189464) Chromatin Immunoprecipitation: TRF-2 Antibody [ABIN1894640] - Analysis in mouse. Titr...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TBPL1 antibody (TBP-Like 1) (ABIN189464) Immunohistochemistry-Paraffin: TRF-2 Antibody [ABIN1894640] - Analysis in xenografted...
Immunofluorescence (IF) image for anti-TBPL1 antibody (TBP-Like 1) (ABIN189464) Immunocytochemistry/Immunofluorescence: TRF-2 Antibody [ABIN1894640] - HeLa cells wer...
Product cited in: Manterola, Sicinski, Wolgemuth: "E-type cyclins modulate telomere integrity in mammalian male meiosis." in: Chromosoma, Vol. 125, Issue 2, pp. 253-64, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Cipressa, Morciano, Bosso, Mannini, Galati, Raffa, Cacchione, Musio, Cenci: "A role for Separase in telomere protection." in: Nature communications, Vol. 7, pp. 10405, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Burla, Carcuro, Torre, Fratini, Crescenzi, DApice, Spitalieri, Raffa, Astrologo, Lattanzi, Cundari, Raimondo, Biroccio, Gatti, Saggio: "The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence." in: Open biology, Vol. 6, Issue 8, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Knecht, Mai: "The Use of 3D Telomere FISH for the Characterization of the Nuclear Architecture in EBV-Positive Hodgkin's Lymphoma." in: Methods in molecular biology (Clifton, N.J.), Vol. 1532, pp. 93-104, 2016 (PubMed).

Shibuya, Hernández-Hernández, Morimoto, Negishi, Höög, Watanabe: "MAJIN Links Telomeric DNA to the Nuclear Membrane by Exchanging Telomere Cap." in: Cell, Vol. 163, Issue 5, pp. 1252-66, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Badie, Carlos, Folio, Okamoto, Bouwman, Jonkers, Tarsounas: "BRCA1 and CtIP promote alternative non-homologous end-joining at uncapped telomeres." in: The EMBO journal, Vol. 34, Issue 3, pp. 410-24, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Ci, Li, Ma, Kong, Zheng, Björkholm, Jia, Xu: "Bortezomib-mediated down-regulation of telomerase and disruption of telomere homeostasis contributes to apoptosis of malignant cells." in: Oncotarget, Vol. 6, Issue 35, pp. 38079-92, 2015 (PubMed). Method employed by authors: Immunohistochemistry (IHC)

Sishc, Nelson, McKenna, Battaglia, Herndon, Idate, Liber, Bailey: "Telomeres and Telomerase in the Radiation Response: Implications for Instability, Reprograming, and Carcinogenesis." in: Frontiers in oncology, Vol. 5, pp. 257, 2015 (PubMed). Method employed by authors: Western Blotting (WB)

Shibuya, Ishiguro, Watanabe: "The TRF1-binding protein TERB1 promotes chromosome movement and telomere rigidity in meiosis." in: Nature cell biology, Vol. 16, Issue 2, pp. 145-56, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Stimpson, Sullivan, Kuo, Sullivan: "Nucleolar organization, ribosomal DNA array stability, and acrocentric chromosome integrity are linked to telomere function." in: PLoS ONE, Vol. 9, Issue 3, pp. e92432, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Chromatin Immunoprecipitation,Immunofluorescence

Arora, Lee, Wischnewski, Brun, Schwarz, Azzalin: "RNaseH1 regulates TERRA-telomeric DNA hybrids and telomere maintenance in ALT tumour cells." in: Nature communications, Vol. 5, pp. 5220, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Chromatin Immunoprecipitation,Immunofluorescence

Wood, Rendtlew Danielsen, Lucas, Rice, Scalzo, Shimi, Goldman, Smith, Le Beau, Kosak: "TRF2 and lamin A/C interact to facilitate the functional organization of chromosome ends." in: Nature communications, Vol. 5, pp. 5467, 2014 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Robin, Ludlow, Batten, Magdinier, Stadler, Wagner, Shay, Wright: "Telomere position effect: regulation of gene expression with progressive telomere shortening over long distances." in: Genes & development, Vol. 28, Issue 22, pp. 2464-76, 2014 (PubMed). (Sample species: Human).

Islam, Thomas, Murty, Sedoris, Miller: "c-Myc quadruplex-forming sequence Pu-27 induces extensive damage in both telomeric and nontelomeric regions of DNA." in: The Journal of biological chemistry, Vol. 289, Issue 12, pp. 8521-31, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Wojdyla, Stone, Sethakorn, Uppada, Devito, Bissonnette, Puri: "T-oligo as an anticancer agent in colorectal cancer." in: Biochemical and biophysical research communications, Vol. 446, Issue 2, pp. 596-601, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Castro-Vega, Jouravleva, Liu, Martinez, Gestraud, Hupé, Servant, Albaud, Gentien, Gad, Richard, Bacchetti, Londoño-Vallejo: "Telomere crisis in kidney epithelial cells promotes the acquisition of a microRNA signature retrieved in aggressive renal cell carcinomas." in: Carcinogenesis, Vol. 34, Issue 5, pp. 1173-80, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Chang, McGhie, Chan, Tang, Anderson, Mann, Andy Choo, Wong: "PML bodies provide an important platform for the maintenance of telomeric chromatin integrity in embryonic stem cells." in: Nucleic acids research, Vol. 41, Issue 8, pp. 4447-58, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Kappei, Butter, Benda, Scheibe, Draškovič, Stevense, Novo, Basquin, Araki, Araki, Krastev, Kittler, Jessberger, Londoño-Vallejo, Mann, Buchholz: "HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment." in: The EMBO journal, Vol. 32, Issue 12, pp. 1681-701, 2013 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Chromatin Immunoprecipitation

Pitman, Wojdyla, Puri: "Mechanism of DNA damage responses induced by exposure to an oligonucleotide homologous to the telomere overhang in melanoma." in: Oncotarget, Vol. 4, Issue 5, pp. 761-71, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Kar, Saha, Purohit, Singh, Kumar, Yadav, Kumar, Thakur, Chowdhury: "Metastases suppressor NME2 associates with telomere ends and telomerase and reduces telomerase activity within cells." in: Nucleic acids research, Vol. 40, Issue 6, pp. 2554-65, 2012 (PubMed).